PMID- 10079412
OWN - NLM
STAT- MEDLINE
DCOM- 19990601
LR  - 20190515
IS  - 1058-0468 (Print)
IS  - 1573-7330 (Electronic)
IS  - 1058-0468 (Linking)
VI  - 16
IP  - 2
DP  - 1999 Feb
TI  - Preimplantation diagnosis by fluorescence in situ hybridization using 13-, 16-, 
      18-, 21-, 22-, X-, and Y-chromosome probes.
PG  - 92-6
AB  - PURPOSE: Our purpose was to select the proper chromosomes for preimplantation 
      diagnosis based on aneuploidy distribution in abortuses and to carry out a 
      feasibility study of preimplantation diagnosis for embryos using multiple-probe 
      fluorescence in situ hybridization (FISH) on the selected chromosomes of biopsied 
      blastomeres. METHODS: After determining the frequency distribution of aneuploidy 
      found in abortuses, seven chromosomes were selected for FISH probes. Blastomeres 
      were obtained from 33 abnormal or excess embryos. The chromosome complements of 
      both the biopsied blastomeres and the remaining sibling blastomeres in each 
      embryo were determined by FISH and compared to evaluate their preimplantation 
      diagnostic potential. RESULTS: Chromosomes (16, 22, X, Y) and (13, 18, 21) were 
      selected on the basis of the high aneuploid prevalence in abortuses for the 
      former group and the presence of trisomy in the newborn for the latter. 
      Thirty-six (72%) of 50 blastomeres gave signals to permit a diagnosis. Diagnoses 
      made from biopsied blastomeres were consistent with the diagnoses made from the 
      remaining sibling blastomeres in 18 embryos. In only 2 of 20 cases did the 
      biopsied blastomere diagnosis and the embryo diagnosis not match. CONCLUSIONS: If 
      FISH of biopsied blastomere was successful, a preimplantation diagnosis could be 
      made with 10% error. When a combination of chromosome-13, -16, -18, -21, -22, -X, 
      and -Y probes was used, up to 65% of the embryos destined to be aborted could be 
      detected.
FAU - Sasabe, Y
AU  - Sasabe Y
AD  - First Department of Obstetrics and Gynecology, School of Medicine, Toho 
      University, Tokyo, Japan.
FAU - Katayama, K P
AU  - Katayama KP
FAU - Nishimura, T
AU  - Nishimura T
FAU - Takahashi, A
AU  - Takahashi A
FAU - Asakura, H
AU  - Asakura H
FAU - Winchester-Peden, K
AU  - Winchester-Peden K
FAU - Wise, L
AU  - Wise L
FAU - Abe, Y
AU  - Abe Y
FAU - Kubo, H
AU  - Kubo H
FAU - Hirakawa, S
AU  - Hirakawa S
LA  - eng
PT  - Clinical Trial
PT  - Controlled Clinical Trial
PT  - Journal Article
PL  - Netherlands
TA  - J Assist Reprod Genet
JT  - Journal of assisted reproduction and genetics
JID - 9206495
RN  - 0 (DNA Probes)
SB  - IM
MH  - Abortion, Spontaneous
MH  - Adult
MH  - Aneuploidy
MH  - Biopsy
MH  - Blastomeres/pathology
MH  - *Chromosomes, Human
MH  - *DNA Probes
MH  - Feasibility Studies
MH  - Female
MH  - Fertilization in Vitro
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Pregnancy
MH  - Preimplantation Diagnosis/*methods
MH  - *X Chromosome
MH  - *Y Chromosome
PMC - PMC3455735
EDAT- 1999/03/18 00:00
MHDA- 1999/03/18 00:01
PMCR- 2000/02/01
CRDT- 1999/03/18 00:00
PHST- 1999/03/18 00:00 [pubmed]
PHST- 1999/03/18 00:01 [medline]
PHST- 1999/03/18 00:00 [entrez]
PHST- 2000/02/01 00:00 [pmc-release]
AID - 421818 [pii]
AID - 10.1023/a:1022520907332 [doi]
PST - ppublish
SO  - J Assist Reprod Genet. 1999 Feb;16(2):92-6. doi: 10.1023/a:1022520907332.