PMID- 10080649
OWN - NLM
STAT- MEDLINE
DCOM- 19990503
LR  - 20190831
IS  - 1387-2273 (Print)
IS  - 1387-2273 (Linking)
VI  - 723
IP  - 1-2
DP  - 1999 Feb 19
TI  - Quantification of 3,4-methylenedioxymetamphetamine and its metabolites in plasma 
      and urine by gas chromatography with nitrogen-phosphorus detection.
PG  - 221-32
AB  - A gas chromatographic method with nitrogen-phosphorus detection involving a 
      solid-liquid extraction phase was developed and validated for the simultaneous 
      quantification of 3,4-methylenedioxymethamphetamine (MDMA) and 
      3,4-methylenedioxyamphetamine (MDA) in plasma. A modification of this method was 
      validated for the analysis of MDMA, MDA, 4-hydroxy-3-methoxymethamphetamine 
      (HMMA) and, 4-hydroxy-3-methoxyamphetamine (HMA) in urine. Under the analytical 
      conditions described, the limits of detection in plasma and urine were less than 
      1.6 microg/l and 47 microg/l, respectively, for all the compounds studied. Good 
      linearity was observed in the concentration range evaluated in plasma (5-400 
      microg/l) and urine (100-2000 microg/l) for all compounds tested. The recoveries 
      obtained from plasma were 85.1% and 91.6% for MDMA and MDA, respectively. Urine 
      recoveries were higher than 90% for MDMA and MDA, 74% for HMMA, and 64% for HMA. 
      Methods have been successfully used in the assessment of plasma and urine 
      concentrations of MDMA and its main metabolites in samples from clinical studies 
      in healthy volunteers.
FAU - Ortuno, J
AU  - Ortuno J
AD  - Pharmacology Research Unit, Institut Municipal d'Investigacio Medica (IMIM), 
      Barcelona, Spain.
FAU - Pizarro, N
AU  - Pizarro N
FAU - Farre, M
AU  - Farre M
FAU - Mas, M
AU  - Mas M
FAU - Segura, J
AU  - Segura J
FAU - Cami, J
AU  - Cami J
FAU - Brenneisen, R
AU  - Brenneisen R
FAU - de la Torre, R
AU  - de la Torre R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Chromatogr B Biomed Sci Appl
JT  - Journal of chromatography. B, Biomedical sciences and applications
JID - 9714109
RN  - 27YLU75U4W (Phosphorus)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Chromatography, Gas/*methods
MH  - Humans
MH  - Male
MH  - N-Methyl-3,4-methylenedioxyamphetamine/blood/*metabolism/urine
MH  - Nitrogen
MH  - Phosphorus
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
EDAT- 1999/03/18 00:00
MHDA- 1999/03/18 00:01
CRDT- 1999/03/18 00:00
PHST- 1999/03/18 00:00 [pubmed]
PHST- 1999/03/18 00:01 [medline]
PHST- 1999/03/18 00:00 [entrez]
AID - 10.1016/s0378-4347(98)00506-4 [doi]
PST - ppublish
SO  - J Chromatogr B Biomed Sci Appl. 1999 Feb 19;723(1-2):221-32. doi: 
      10.1016/s0378-4347(98)00506-4.