PMID- 10082778
OWN - NLM
STAT- MEDLINE
DCOM- 19990402
LR  - 20180330
IS  - 0022-3166 (Print)
IS  - 0022-3166 (Linking)
VI  - 129
IP  - 3
DP  - 1999 Mar
TI  - Calcium does not inhibit iron absorption or alter iron status in infant piglets 
      adapted to a high calcium diet.
PG  - 707-11
AB  - The purpose of this study was to investigate whether a dietary calcium:iron ratio 
      similar to that often consumed by premature human infants inhibits iron 
      absorption in infant piglets adapted to a high calcium diet. Male Yorkshire 
      piglets were randomized at 3 to 4 d of age to a high calcium diet (4.67 g/L = HC) 
      or a normal calcium diet (2.0 g/L = NC) and fed for 2 to 2.5 wk. An iron dextran 
      injection was administered in amounts to achieve a marginal state of iron 
      repletion to simulate iron status of premature infants. In vivo iron absorption 
      from the diet was determined using the radiotracers 55Fe and 59Fe and whole body 
      counting. Calcium:iron interactions at absorption sites in piglets fed HC and NC 
      were investigated by measurements of time-dependent 59Fe uptake in response to 
      different calcium:iron ratios in vitro in brush border membrane vesicles (BBMV). 
      In vivo iron absorption from the diet did not differ between NC and HC diet 
      groups [57 +/- 8% versus 55 +/- 17% (mean +/- SD), respectively]. Iron status and 
      iron contencentrations in spleen, liver, intestine, kidney and heart did not 
      differ between diet groups. Iron uptake in BBMV was significantly reduced by 
      calcium in both HC and NC (P < 0.001); but there were no significant differences 
      in iron uptake in response to different calcium:iron ratios between HC and NC. 
      With feeding a HC diet for 2 wk there may be an adaptive response to counteract 
      the inhibitory effects of calcium on iron absorption, thus resulting in similar 
      in vivo iron absorption and iron status irrespective of the 1.3-fold difference 
      in dietary calcium:iron ratio between piglet groups. However, future studies are 
      needed to determine the specific sites of calcium:iron interactions and 
      adaptation mechanisms. Since the calcium:iron ratios used in this study reflect 
      the usual calcium:iron ratios in diets for premature infants, it is unlikely that 
      interactive effects of calcium with iron will compromise iron status in this 
      infant population when diets are supplemented with calcium.
FAU - Wauben, I P
AU  - Wauben IP
AD  - Department of Pediatrics, McMaster University, Hamilton ON L8N 3Z5, Canada.
FAU - Atkinson, S A
AU  - Atkinson SA
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Nutr
JT  - The Journal of nutrition
JID - 0404243
RN  - E1UOL152H7 (Iron)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Animals, Newborn/*metabolism
MH  - Calcium/*administration & dosage/pharmacology
MH  - *Diet
MH  - Drug Interactions
MH  - Humans
MH  - In Vitro Techniques
MH  - Intestinal Absorption/*drug effects
MH  - Intestines/ultrastructure
MH  - Iron/administration & dosage/blood/*metabolism
MH  - Male
MH  - Microvilli/drug effects/metabolism
MH  - *Nutritional Status
MH  - Swine
MH  - Weight Gain
EDAT- 1999/03/20 00:00
MHDA- 1999/03/20 00:01
CRDT- 1999/03/20 00:00
PHST- 1999/03/20 00:00 [pubmed]
PHST- 1999/03/20 00:01 [medline]
PHST- 1999/03/20 00:00 [entrez]
AID - 10.1093/jn/129.3.707 [doi]
PST - ppublish
SO  - J Nutr. 1999 Mar;129(3):707-11. doi: 10.1093/jn/129.3.707.