PMID- 10082829
OWN - NLM
STAT- MEDLINE
DCOM- 19990506
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 818
IP  - 2
DP  - 1999 Feb 13
TI  - Neurotrophins promote regeneration of sensory axons in the adult rat spinal cord.
PG  - 431-8
AB  - We have investigated the effects of nerve growth factor (NGF), brain-derived 
      neurotrophic factor (BDNF), and neurotrophin-3 (NT-3) on the intraspinal 
      regeneration of anterogradely labeled axotomized ascending primary sensory fibers 
      in the adult rat. These fibers were allowed to grow across a predegenerated 
      peripheral nerve graft and back into the thoracic spinal cord. In control animals 
      that had been infused with vehicle for two weeks into the dorsal column, 3 mm 
      rostral to the nerve graft, essentially no fibers had extended from the nerve 
      graft back into the spinal cord. The number of sensory fibers in the rostral end 
      of the nerve graft was not significantly different between control and 
      neurotrophin-infused animals. With infusion of NGF, 37+/-2% of the fibers at the 
      rostral end of the graft had grown up to 0.5 mm into the dorsal column white 
      matter, 30+/-2% up to 1 mm, 19+/-3% up to 2 mm and 8+/-2% up to 3 mm, i.e., the 
      infusion site. With infusion of NT-3, sensory fiber outgrowth was similar to that 
      seen with NGF, but with BDNF fewer fibers reached farther distances into the 
      cord. Infusion of a mixture of all three neurotrophins did not increase the 
      number of regenerating sensory fibers above that seen after infusion of the 
      individual neurotrophins. These findings suggest that injured ascending sensory 
      axons are responsive to all three neurotrophins and confirm our previous findings 
      that neurotrophic factors can promote regeneration in the adult central nervous 
      system.
CI  - Copyright 1999 Elsevier Science B.V.
FAU - Oudega, M
AU  - Oudega M
AD  - The Miami Project to Cure Paralysis, University of Miami School of Medicine, PO 
      Box 016960, R-48, Miami, FL 33136, USA. moudega@miamiproj.med.miami.edu
FAU - Hagg, T
AU  - Hagg T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neurotrophin 3)
SB  - IM
MH  - Animals
MH  - Axons/*drug effects
MH  - Axotomy
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Female
MH  - Ganglia, Sensory/*drug effects/ultrastructure
MH  - Nerve Growth Factors/*pharmacology
MH  - Nerve Regeneration/*drug effects
MH  - Neurotrophin 3
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spinal Cord/*drug effects/ultrastructure
EDAT- 1999/03/20 00:00
MHDA- 1999/03/20 00:01
CRDT- 1999/03/20 00:00
PHST- 1999/03/20 00:00 [pubmed]
PHST- 1999/03/20 00:01 [medline]
PHST- 1999/03/20 00:00 [entrez]
AID - S0006-8993(98)01314-6 [pii]
AID - 10.1016/s0006-8993(98)01314-6 [doi]
PST - ppublish
SO  - Brain Res. 1999 Feb 13;818(2):431-8. doi: 10.1016/s0006-8993(98)01314-6.