PMID- 10083791
OWN - NLM
STAT- MEDLINE
DCOM- 19990421
LR  - 20090730
IS  - 0970-258X (Print)
IS  - 0970-258X (Linking)
VI  - 11
IP  - 6
DP  - 1998 Nov-Dec
TI  - Detection of chromosomal abnormalities using fluorescence in situ hybridization 
      (FISH).
PG  - 259-63
AB  - BACKGROUND: A number of studies have demonstrated the use of molecular 
      cytogenetic techniques for clinical diagnosis. We compared the results of FISH 
      analysis and conventional cytogenetics on different tissue samples for detection 
      of chromosomal aberrations and to assess the utility of FISH assay for clinical 
      diagnosis. METHODS: Karyotypic analysis was carried out on 50 samples--20 
      peripheral blood samples, 20 bone marrow samples and 10 prenatal (chorionic 
      villi/amniotic fluid) samples. The same chromosome preparations were further 
      subjected to FISH analysis using probes specific for chromosome X, Y, 21 or 
      bcr-abl gene. RESULTS: The results of FISH analysis were in conformity with the 
      cytogenetic results in all the samples except one. FISH analysis could reveal 
      hybridization signals even on poorly spread metaphase chromosomes and interphase 
      nuclei. It was also possible to detect subtle chromosomal aberrations which were 
      not detected using conventional chromosomal analysis. CONCLUSION: FISH is a 
      powerful, sensitive molecular cytogenetic technique which can be used as an 
      adjunct to conventional chromosomal analysis for prenatal diagnosis and the 
      diagnosis and management of cancer patients. FISH analysis should be used as a 
      supplement to conventional cytogenetics.
FAU - Jobanputra, V
AU  - Jobanputra V
AD  - All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
FAU - Kriplani, A
AU  - Kriplani A
FAU - Choudhry, V P
AU  - Choudhry VP
FAU - Kucheria, K
AU  - Kucheria K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - India
TA  - Natl Med J India
JT  - The National medical journal of India
JID - 8809315
SB  - IM
MH  - Amniocentesis
MH  - Chorionic Villi Sampling
MH  - *Chromosome Aberrations/*diagnosis
MH  - *Chromosome Disorders
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Neoplasms/genetics
MH  - Pregnancy
MH  - Prenatal Diagnosis
EDAT- 1999/03/20 00:00
MHDA- 1999/03/20 00:01
CRDT- 1999/03/20 00:00
PHST- 1999/03/20 00:00 [pubmed]
PHST- 1999/03/20 00:01 [medline]
PHST- 1999/03/20 00:00 [entrez]
PST - ppublish
SO  - Natl Med J India. 1998 Nov-Dec;11(6):259-63.