PMID- 10085261
OWN - NLM
STAT- MEDLINE
DCOM- 19990719
LR  - 20220215
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 112 ( Pt 8)
DP  - 1999 Apr
TI  - Locomotory behaviour of epitheliocytes and fibroblasts on metallic grids.
PG  - 1273-82
AB  - Behaviour of epitheliocytes and fibroblasts on special discontinuous substrata 
      (metallic grids with square openings of 45x45 microm2) was examined in order to 
      compare the ability of these cells to spread in two mutually perpendicular 
      directions and to stretch over the void spaces. Two cell types with typical 
      fibroblastic morphology, the AGO 1523 line of human foreskin fibroblasts and 
      secondary cultures of mouse embryo fibroblasts, and three cell types with typical 
      epithelial morphology, primary mouse hepatocytes, the IAR-2 line of rat liver 
      cells and the MDCK line of canine kidney epithelial cells (clone 20) were used. 
      We also examined the epitheliocytes (MDCK cells, clone 20) transformed to 
      fibroblast-like morphology by treatment with hepatocyte growth factor/scatter 
      factor (HGF/SF). Time-lapse video microscopy, scanning electron microscopy and 
      immunofluorescence microscopy were used to examine cell reorganizations at 
      various stages of spreading. It was found that early stages of spreading of 
      fibroblasts and epitheliocytes were similar: the cell spread along two bars, 
      perpendicular to each other (bar and crossbar), with the formation of a small 
      triangular lamellar cytoplasm stretched over the opening. Later central parts of 
      the bodies of the fibroblasts retracted from the bars so that the cells remained 
      attached only by their polar lamellae. Successive expansions and partial 
      retractions of these lamellae led to elongation of the cell body crossing several 
      openings of the grid. Epitheliocytes, in contrast to fibroblasts, at the late 
      stages of spreading did not retract their bodies and did not contract polar 
      lamellae. As a result, their central lamellae stretched progressively over the 
      openings. As a result of the treatment of MDCK epitheliocytes with HGF/SF the 
      behaviour of the cells on the grids became similar to that of fibroblasts. It is 
      suggested that these distinct spreading patterns of epitheliocytes and 
      fibroblasts are due to the type-specific differences in the actin-myosin cortex. 
      Experiments with microtubule-specific drugs, colcemid and taxol, indicate that 
      the organization of this cortex is under microtubular control.
FAU - Rovensky, Y A
AU  - Rovensky YA
AD  - Cancer Research Center of the Russian Federation, Moscow, Russia.
FAU - Domnina, L V
AU  - Domnina LV
FAU - Ivanova, O Y
AU  - Ivanova OY
FAU - Vasiliev, J M
AU  - Vasiliev JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (Actins)
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Fibronectins)
RN  - 0 (Tubulin)
RN  - 125361-02-6 (Vinculin)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 789U1901C5 (Copper)
RN  - 7OV03QG267 (Nickel)
RN  - P88XT4IS4D (Paclitaxel)
RN  - Z01IVE25KI (Demecolcine)
SB  - IM
MH  - Actin Cytoskeleton/metabolism
MH  - Actins/metabolism
MH  - Animals
MH  - Antineoplastic Agents, Phytogenic/pharmacology
MH  - Cell Culture Techniques/*instrumentation
MH  - Cell Movement
MH  - Cells, Cultured
MH  - Copper/metabolism
MH  - Demecolcine/pharmacology
MH  - Dogs
MH  - Epithelial Cells/*physiology
MH  - Fibroblasts/*physiology
MH  - Fibronectins/metabolism
MH  - Hepatocyte Growth Factor/pharmacology
MH  - Humans
MH  - Mice
MH  - Microscopy, Electron, Scanning
MH  - Microscopy, Fluorescence
MH  - Microscopy, Video
MH  - Nickel/metabolism
MH  - Paclitaxel/pharmacology
MH  - Rats
MH  - Time Factors
MH  - Tubulin/metabolism
MH  - Vinculin/metabolism
EDAT- 1999/03/23 00:00
MHDA- 1999/03/23 00:01
CRDT- 1999/03/23 00:00
PHST- 1999/03/23 00:00 [pubmed]
PHST- 1999/03/23 00:01 [medline]
PHST- 1999/03/23 00:00 [entrez]
AID - 10.1242/jcs.112.8.1273 [doi]
PST - ppublish
SO  - J Cell Sci. 1999 Apr;112 ( Pt 8):1273-82. doi: 10.1242/jcs.112.8.1273.