PMID- 10085341
OWN - NLM
STAT- MEDLINE
DCOM- 19990506
LR  - 20171213
IS  - 0022-3077 (Print)
IS  - 0022-3077 (Linking)
VI  - 81
IP  - 3
DP  - 1999 Mar
TI  - Increased intracellular calcium in rat anterior piriform cortex in response to 
      threonine after threonine deprivation.
PG  - 1147-9
AB  - The anterior piriform cortex (APC) may serve as the chemosensor for amino acid 
      (AA) deficiency in rats. To investigate the mechanism by which the APC recognizes 
      a limiting indispensable AA (IAA), we examined changes in intracellular calcium 
      ([Ca2+]i) in APC slices after culture in medium with or without threonine (Thr) 
      or lysine (Lys). The addition of 1 or 10 mM Thr to slices previously incubated in 
      Thr-devoid medium resulted in a significant and sustained increase in [Ca2+]i 
      compared to control slices; an effect not seen when isoleucine, another IAA, was 
      added. Similar results were seen when lysine, but not threonine, was added to 
      slices incubated in lysine-devoid medium. The rise in [Ca2+]i resulting from the 
      addition of the limiting IAA to deficient slices may be linked to enhanced 
      activity of the appropriate AA transporter. This is suggested by preliminary 
      findings that serine, a small neutral AA that uses the same transporter as 
      threonine, gave rise to an enhanced response in the Thr-deficient slice.
FAU - Magrum, L J
AU  - Magrum LJ
AD  - Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine 
      and Food Intake Laboratory, University of California, Davis, California 95616, 
      USA.
FAU - Hickman, M A
AU  - Hickman MA
FAU - Gietzen, D W
AU  - Gietzen DW
LA  - eng
GR  - DK-35747/DK/NIDDK NIH HHS/United States
GR  - DK-50347/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurophysiol
JT  - Journal of neurophysiology
JID - 0375404
RN  - 0 (Culture Media)
RN  - 2ZD004190S (Threonine)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Calcium/*metabolism
MH  - Cell Communication/drug effects
MH  - Cerebral Cortex/*drug effects/metabolism
MH  - Culture Media
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Threonine/deficiency/*pharmacology
EDAT- 1999/03/23 00:00
MHDA- 1999/03/23 00:01
CRDT- 1999/03/23 00:00
PHST- 1999/03/23 00:00 [pubmed]
PHST- 1999/03/23 00:01 [medline]
PHST- 1999/03/23 00:00 [entrez]
AID - 10.1152/jn.1999.81.3.1147 [doi]
PST - ppublish
SO  - J Neurophysiol. 1999 Mar;81(3):1147-9. doi: 10.1152/jn.1999.81.3.1147.