PMID- 10086076
OWN - NLM
STAT- MEDLINE
DCOM- 19990415
LR  - 20170404
IS  - 1354-4195 (Print)
IS  - 1354-4195 (Linking)
VI  - 3
IP  - 4
DP  - 1997 Dec
TI  - Human endometrial decidual cell-associated 11 beta-hydroxysteroid dehydrogenase 
      expression: its potential role in implantation.
PG  - 259-64
AB  - During pregnancy excess corticosteroid exposure can disturb the normal pattern of 
      growth and differentiation of the primate fetus. This is normally prevented by 
      the action of 11 beta-hydroxysteroid dehydrogenase (11 beta-HSD), which converts 
      cortisol to its biologically inactive 11-oxo form, thereby ensuring that little 
      or no cortisol is transferred to the fetus. During implantation, extravillous 
      trophoblasts breech uterine vessels that are embedded in a decidual cell matrix. 
      Through this invasive process the embryo gains requisite access to the maternal 
      blood supply, while risking exposure to high circulating glucocorticoid levels. 
      Thus, the expression of 11 beta-HSD by the decidual cell layer may be essential 
      in regulating cortisol exposure of the developing embryo prior to placentation. 
      In order to investigate the potential contribution of decidual cells to 
      glucocorticoid metabolism, we evaluated the expression of both known 11 beta-HSD 
      isoforms, 11 beta-HSD1, whose catalytic activity is NADP(+)-dependent, and 
      NAD(+)-dependent 11 beta-HSD2, during decidualization of monolayers of human 
      endometrial stromal cells. The differential actions of ovarian steroids on human 
      endometrium are simulated in this in vitro model. Thus, progestins induce the 
      expression of several decidualization markers in the cultured stromal cells, and 
      consistent with its priming action in vivo, estradiol augments this expression. 
      The results of our studies established a link between in vitro decidualization 
      and enhanced glucocorticoid metabolizing capacity. Accordingly, the catalytic 
      activities of both 11 beta-HSD isoforms were enhanced by incubation of the 
      precursor stromal cells with medroxyprogesterone acetate, and further enhanced by 
      estradiol, despite a lack of response to estradiol alone. This differential 
      response to estradiol and progestin was reflected in parallel changes in steady 
      state levels of 11 beta-HSD1 messenger RNA. The role of glucocorticoid 
      metabolizing activity of the decidual cell is discussed in terms of its 
      implications in determining the exposure of the implanting embryo to biologically 
      active glucocorticoids.
FAU - Arcuri, F
AU  - Arcuri F
AD  - Institute of Pathologic Anatomy and Histology, New York University Medical Center 
      10016, USA.
FAU - Battistini, S
AU  - Battistini S
FAU - Hausknecht, V
AU  - Hausknecht V
FAU - Cintorino, M
AU  - Cintorino M
FAU - Lockwood, C J
AU  - Lockwood CJ
FAU - Schatz, F
AU  - Schatz F
LA  - eng
GR  - R29 HD29540/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Early Pregnancy
JT  - Early pregnancy : biology and medicine : the official journal of the Society for 
      the Investigation of Early Pregnancy
JID - 9508879
RN  - 0 (Isoenzymes)
RN  - 4TI98Z838E (Estradiol)
RN  - 63231-63-0 (RNA)
RN  - EC 1.1.- (Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenases)
RN  - FO4V44A3G3 (11-dehydrocorticosterone)
RN  - HSU1C9YRES (Medroxyprogesterone)
RN  - W980KJ009P (Corticosterone)
SB  - IM
MH  - 11-beta-Hydroxysteroid Dehydrogenases
MH  - Blotting, Northern
MH  - Chromatography, Thin Layer
MH  - Corticosterone/analogs & derivatives/analysis/metabolism
MH  - Decidua/*enzymology/metabolism
MH  - Embryo Implantation/*physiology
MH  - Estradiol/metabolism
MH  - Female
MH  - *Gene Expression Regulation, Developmental
MH  - Humans
MH  - Hydroxysteroid Dehydrogenases/*genetics/physiology
MH  - Isoenzymes
MH  - Medroxyprogesterone/metabolism
MH  - Pregnancy
MH  - RNA/chemistry
MH  - Stromal Cells/enzymology
EDAT- 1999/03/23 00:00
MHDA- 1999/03/23 00:01
CRDT- 1999/03/23 00:00
PHST- 1999/03/23 00:00 [pubmed]
PHST- 1999/03/23 00:01 [medline]
PHST- 1999/03/23 00:00 [entrez]
PST - ppublish
SO  - Early Pregnancy. 1997 Dec;3(4):259-64.