PMID- 10086800
OWN - NLM
STAT- MEDLINE
DCOM- 19990415
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 104
IP  - 3
DP  - 1999 Mar
TI  - The incidence of trisomy 3 in splenic lymphoma with villous lymphocytes: a study 
      by FISH.
PG  - 600-4
AB  - Splenic lymphoma with villous lymphocytes (SLVL) is a low-grade B-cell 
      lymphoproliferative disorder characterized by splenomegaly and circulating 
      villous lymphocytes. The relationship between SLVL and splenic marginal zone 
      lymphoma (SMZL), a disorder with identical splenic histology to SLVL, is not 
      clear. Previous studies have failed to show a consistent karyotypic abnormality 
      in SLVL whereas trisomy 3 has been reported in patients with SMZL. The presence 
      of trisomy 3 in SMZL and its absence in SLVL has been viewed as evidence that 
      these are different diseases. However, it is possible that the frequency of 
      trisomy 3 in SLVL has been underestimated because previous studies have relied on 
      conventional cytogenetics. We have therefore used interphase fluorescence in situ 
      hybridization (FISH) to re-assess the frequency of trisomy 3 in SLVL. We studied 
      70 patients, who were stratified into four groups according to the percentage of 
      circulating villous lymphocytes. Trisomy 3 was found overall in 17% of patients. 
      In particular, trisomy 3 was detected in 13% of cases with >50% of villous 
      lymphocytes and which were considered typical of SLVL. In conclusion, we have 
      demonstrated that some patients with SLVL have circulating cells with trisomy 3, 
      which does not support the view that SLVL and SMZL are different diseases on the 
      basis of the incidence of trisomy 3.
FAU - Gruszka-Westwood, A M
AU  - Gruszka-Westwood AM
AD  - Academic Department of Haematology and Cytogenetics, Institute of Cancer 
      Research/Royal Marsden NHS Trust, London.
FAU - Matutes, E
AU  - Matutes E
FAU - Coignet, L J
AU  - Coignet LJ
FAU - Wotherspoon, A
AU  - Wotherspoon A
FAU - Catovsky, D
AU  - Catovsky D
LA  - eng
PT  - Journal Article
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
SB  - IM
MH  - Aged
MH  - Chromosomes, Human, Pair 3/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase
MH  - Lymphoma/*genetics
MH  - Male
MH  - Sensitivity and Specificity
MH  - Splenic Neoplasms/*genetics
MH  - *Trisomy
EDAT- 1999/03/23 00:00
MHDA- 1999/03/23 00:01
CRDT- 1999/03/23 00:00
PHST- 1999/03/23 00:00 [pubmed]
PHST- 1999/03/23 00:01 [medline]
PHST- 1999/03/23 00:00 [entrez]
AID - 10.1046/j.1365-2141.1999.01209.x [doi]
PST - ppublish
SO  - Br J Haematol. 1999 Mar;104(3):600-4. doi: 10.1046/j.1365-2141.1999.01209.x.