PMID- 10087166
OWN - NLM
STAT- MEDLINE
DCOM- 19990521
LR  - 20191210
IS  - 0003-9861 (Print)
IS  - 0003-9861 (Linking)
VI  - 364
IP  - 1
DP  - 1999 Apr 1
TI  - Effects of docosahexaenoic and arachidonic acids on the synthesis and 
      distribution of aminophospholipids during neuronal differentiation of PC12 cells.
PG  - 67-74
AB  - We have shown previously that docosahexaenoic acid (DHA) promotes and arachidonic 
      acid (AA) suppresses neurite outgrowth of PC12 cells induced by nerve growth 
      factor (NGF) and that incorporation of [3H]ethanolamine into 
      phosphatidylethanolamine (PE) is suppressed in PC12 cells by AA while DHA has no 
      effect. In the present study, the effects of these fatty acids on PE synthesis 
      via decarboxylation of phosphatidylserine (PS), another pathway of PE synthesis, 
      and distribution of aminophospholipids were examined. Incorporation of [3H]serine 
      into PS and PE was elevated in the course of NGF-induced differentiation and was 
      further stimulated significantly by DHA, but not by AA. [3H]Ethanolamine uptake 
      by PC12 cells was significantly suppressed by AA but not by DHA while these fatty 
      acids did not affect [3H]serine uptake, indicating that the suppression by AA of 
      [3H]ethanolamine incorporation into phosphatidylethanolamine is attributable, at 
      least in part, to a reduction in [3H]ethanolamine uptake. The distribution of PE 
      in the outer leaflet of plasma membrane decreased during differentiation, which 
      is known to be accompanied by an increase in the surface area of plasma membrane. 
      Supplementation of PC12 cells with DHA or AA did not affect the distribution of 
      aminophospholipids. Thus, DHA and AA affected aminophospholipid synthesis and 
      neurite outgrowth differently, but not the transport and distribution of 
      aminophospholipids, while the PE concentration in the outer leaflet of the plasma 
      membrane decreased in association with morphological changes in PC12 cells 
      induced by NGF.
CI  - Copyright 1999 Academic Press.
FAU - Ikemoto, A
AU  - Ikemoto A
AD  - Faculty of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabedori, 
      Nagoya, Mizuhoku, 467-8603, Japan. aikemoto@phar.nagoya-cu.ac.jp
FAU - Kobayashi, T
AU  - Kobayashi T
FAU - Emoto, K
AU  - Emoto K
FAU - Umeda, M
AU  - Umeda M
FAU - Watanabe, S
AU  - Watanabe S
FAU - Okuyama, H
AU  - Okuyama H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arch Biochem Biophys
JT  - Archives of biochemistry and biophysics
JID - 0372430
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Hemolysin Proteins)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Peptides)
RN  - 0 (Peptides, Cyclic)
RN  - 0 (Phosphatidylethanolamines)
RN  - 0 (Phosphatidylserines)
RN  - 0 (Phospholipids)
RN  - 10028-17-8 (Tritium)
RN  - 110655-58-8 (lanthiopeptin)
RN  - 25167-62-8 (Docosahexaenoic Acids)
RN  - 27YG812J1I (Arachidonic Acid)
RN  - 452VLY9402 (Serine)
RN  - 5KV86114PT (Ethanolamine)
RN  - 8T3HQG2ZC4 (Trinitrobenzenesulfonic Acid)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/metabolism
MH  - Arachidonic Acid/*pharmacology
MH  - Cell Differentiation
MH  - Cell Membrane/metabolism
MH  - Cell Size/drug effects
MH  - Docosahexaenoic Acids/*pharmacology
MH  - Ethanolamine/metabolism
MH  - Flow Cytometry
MH  - Hemolysin Proteins/metabolism
MH  - Nerve Growth Factors/pharmacology
MH  - Neurons/drug effects/*metabolism
MH  - PC12 Cells
MH  - *Peptides
MH  - Peptides, Cyclic
MH  - Phosphatidylethanolamines/metabolism
MH  - Phosphatidylserines/metabolism
MH  - Phospholipids/*metabolism
MH  - Rats
MH  - Serine/metabolism
MH  - Trinitrobenzenesulfonic Acid/metabolism
MH  - Tritium
EDAT- 1999/03/24 00:00
MHDA- 1999/03/24 00:01
CRDT- 1999/03/24 00:00
PHST- 1999/03/24 00:00 [pubmed]
PHST- 1999/03/24 00:01 [medline]
PHST- 1999/03/24 00:00 [entrez]
AID - S0003-9861(99)91110-4 [pii]
AID - 10.1006/abbi.1999.1110 [doi]
PST - ppublish
SO  - Arch Biochem Biophys. 1999 Apr 1;364(1):67-74. doi: 10.1006/abbi.1999.1110.