PMID- 10087940
OWN - NLM
STAT- MEDLINE
DCOM- 19990407
LR  - 20220331
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 109
IP  - 2
DP  - 1999 Mar
TI  - Fluorescence in situ hybridization analysis shows the frequent occurrence of 
      14q32.3 rearrangements with involvement of immunoglobulin switch regions in 
      myeloma cell lines.
PG  - 99-107
AB  - In many B-cell malignancies, 14q32.3 chromosomal rearrangements involving the 
      immunoglobulin heavy chain (IgH) locus have been shown to be pathognomonic for 
      the disease. Although in myeloma heterogeneous and complex karyotypes are found, 
      14q32.3 translocations are prominent. However, owing to the telomeric position of 
      the IgH locus, 14q32.3 translocations may be easily missed. We established 
      fluorescence in situ hybridization (FISH) assays on chromosomes and DNA fibers to 
      determine both the occurrence of 14q32.3 rearrangements in myeloma cell lines and 
      the precise localization of the breakpoints in the IgH locus. Our results show 
      that 14q32.3 chromosomal rearrangements are present in almost every myeloma cell 
      line analyzed (17 of 19, 89%). Breakpoint analysis of the lines harboring one or 
      more 14q32.3 rearrangements with the use of fiber-FISH revealed the involvement 
      of switch regions in the IgH locus in 11 of 17 cell lines. Remarkably, 
      pseudogamma genes without switch regions were involved in 3 of 17 cell lines, all 
      derived from IgA myelomas. Three of 17 cell lines contained breakpoints outside a 
      switch or immunoglobulin heavy chain constant region. The almost ubiquitous 
      presence of 14q32.3 rearrangements suggests an obligatory role in the development 
      of myeloma. The high incidence of breakpoints involving switch regions indicates 
      an oncogenic event in a late stage of B-cell differentiation.
FAU - Kuipers, J
AU  - Kuipers J
AD  - Department of Immunology, Utrecht University, The Netherlands.
FAU - Vaandrager, J W
AU  - Vaandrager JW
FAU - Weghuis, D O
AU  - Weghuis DO
FAU - Pearson, P L
AU  - Pearson PL
FAU - Scheres, J
AU  - Scheres J
FAU - Lokhorst, H M
AU  - Lokhorst HM
FAU - Clevers, H
AU  - Clevers H
FAU - Bast, B J
AU  - Bast BJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Immunoglobulin Heavy Chains)
SB  - IM
MH  - Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 14
MH  - Gene Frequency
MH  - Humans
MH  - Immunoglobulin Class Switching/*genetics
MH  - Immunoglobulin Heavy Chains/biosynthesis/genetics
MH  - *In Situ Hybridization, Fluorescence
MH  - Metaphase
MH  - Multiple Myeloma/*genetics/pathology
MH  - Tumor Cells, Cultured
EDAT- 1999/03/24 00:00
MHDA- 1999/03/24 00:01
CRDT- 1999/03/24 00:00
PHST- 1999/03/24 00:00 [pubmed]
PHST- 1999/03/24 00:01 [medline]
PHST- 1999/03/24 00:00 [entrez]
AID - S0165460898001575 [pii]
AID - 10.1016/s0165-4608(98)00157-5 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1999 Mar;109(2):99-107. doi: 
      10.1016/s0165-4608(98)00157-5.