PMID- 10088602
OWN - NLM
STAT- MEDLINE
DCOM- 19990325
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 65
IP  - 2
DP  - 1999 Feb
TI  - Activation of the granule pool of the NADPH oxidase accelerates apoptosis in 
      human neutrophils.
PG  - 196-204
AB  - Oxidative stress induces apoptosis in many types of cells, including human 
      neutrophils. Our objective was to determine whether reactive oxygen species (ROS) 
      produced by activated neutrophils are associated with accelerated apoptosis. 
      Exposing neutrophils to ionomycin or phorbol myristate acetate (PMA) induced 
      intracellular H2O2 production and rapid onset of apoptosis, measured as condensed 
      chromatin, cellular shrinkage, and DNA fragmentation. Neutrophils activated with 
      formyl-methionyl-leucyl-phenylalanine (fMLP) generated mainly extracellular H2O2 
      and did not undergo apoptosis. Exogenously added H2O2, together with the catalase 
      blocker sodium azide, induced apoptosis to the same extent and with similar 
      kinetics as PMA and ionomycin. Adenosine inhibited ionomycin-induced 
      intracellular H2O2 production and apoptosis. Neither PMA nor ionomycin caused 
      apoptosis in dimethyl sulfoxide-differentiated HL-60 cells, which are incapable 
      of intracellular H2O2 production, whereas H2O2 induced apoptosis more efficiently 
      in these cells than in neutrophils. We propose that activated neutrophils use 
      intracellularly formed H2O2 to commit suicide.
FAU - Lundqvist-Gustafsson, H
AU  - Lundqvist-Gustafsson H
AD  - Division of Pathology II, Faculty of Health Sciences, Linkoping University, 
      Sweden. helen.lundqvist@pod.liu.se
FAU - Bengtsson, T
AU  - Bengtsson T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Ionophores)
RN  - 53-59-8 (NADP)
RN  - 56092-81-0 (Ionomycin)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Apoptosis/genetics/*physiology
MH  - DNA Fragmentation
MH  - Enzyme Activation
MH  - HL-60 Cells/drug effects/metabolism
MH  - Humans
MH  - Hydrogen Peroxide/metabolism/pharmacology
MH  - Ionomycin/pharmacology
MH  - Ionophores/pharmacology
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - NADP/*metabolism
MH  - Neutrophil Activation
MH  - Neutrophils/drug effects/enzymology/*physiology
MH  - Oxidative Stress/physiology
MH  - Tetradecanoylphorbol Acetate/pharmacology
EDAT- 1999/03/24 00:00
MHDA- 1999/03/24 00:01
CRDT- 1999/03/24 00:00
PHST- 1999/03/24 00:00 [pubmed]
PHST- 1999/03/24 00:01 [medline]
PHST- 1999/03/24 00:00 [entrez]
AID - 10.1002/jlb.65.2.196 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1999 Feb;65(2):196-204. doi: 10.1002/jlb.65.2.196.