PMID- 1009000
OWN - NLM
STAT- MEDLINE
DCOM- 19770331
LR  - 20181113
IS  - 0007-1021 (Print)
IS  - 0007-1021 (Linking)
VI  - 57
IP  - 6
DP  - 1976 Dec
TI  - Skin reaction and antibody responses in guinea-pigs sensitized to human leukaemia 
      cells or their nuclei in combination with Bacillus Calmette-Guerin.
PG  - 706-12
AB  - Guinea-pigs sensitized by subcutaneous injection of chronic lymphatic leukaemia 
      (CLL) cells combined with Bacillus Calmette-Guerin (BCG) displayed good skin 
      reacitons 24 and 48 h after challenge with CLL cells. Equally good responses were 
      also demonstrated using nuclei from the leukaemic cells in combination with BCG. 
      These reactions were significantly greater than those produced in the same manner 
      but without BCG. Sera form the animals were examined for the presence of 
      antibodies against CLL cells by cytotoxicity and immunofluorescence techniques. 
      Only samples from guinea-pigs innoculated with CLL cells were found to contain 
      significant antibodies. Histological examination showed that whereas leukaemic 
      cells persisted at the sensitizing injection site leukaemic cell nuclei could not 
      be visualized. It is suggested that because leukaemic cell nuclei in combination 
      with BCG are able to induce good skin reactivity without provoking a vigorous 
      humoral antibody response they may have possible advantages over leukaemic cells 
      when used for immunotherapy.
FAU - Barnes, R M
AU  - Barnes RM
FAU - Lewis, C M
AU  - Lewis CM
FAU - Pegrum, G D
AU  - Pegrum GD
FAU - Prince, G H
AU  - Prince GH
LA  - eng
PT  - Journal Article
PL  - England
TA  - Br J Exp Pathol
JT  - British journal of experimental pathology
JID - 0372543
RN  - 0 (BCG Vaccine)
SB  - IM
MH  - Animals
MH  - *Antibody Formation
MH  - BCG Vaccine/*pharmacology
MH  - Cell Nucleus/immunology
MH  - Cytotoxicity Tests, Immunologic
MH  - Guinea Pigs
MH  - Leukemia, Lymphoid/*immunology
MH  - Male
MH  - Skin Tests
PMC - PMC2041245
EDAT- 1976/12/01 00:00
MHDA- 1976/12/01 00:01
PMCR- 1976/12/01
CRDT- 1976/12/01 00:00
PHST- 1976/12/01 00:00 [pubmed]
PHST- 1976/12/01 00:01 [medline]
PHST- 1976/12/01 00:00 [entrez]
PHST- 1976/12/01 00:00 [pmc-release]
PST - ppublish
SO  - Br J Exp Pathol. 1976 Dec;57(6):706-12.