PMID- 10090536
OWN - NLM
STAT- MEDLINE
DCOM- 19990517
LR  - 20190706
IS  - 0009-8981 (Print)
IS  - 0009-8981 (Linking)
VI  - 280
IP  - 1-2
DP  - 1999 Feb
TI  - Levels of free prostate-specific antigen (PSA) can be selectively measured by 
      heat treatment of serum: free/total-PSA ratios improve detection of prostate 
      carcinoma.
PG  - 195-203
AB  - We studied a simple heat treatment method for measuring free prostate-specific 
      antigen (PSA). Samples were incubated at 56, 58, and 60 degrees C for 5, 15, 30, 
      45, and 60 min. Then, 1 ml samples were fractionated on a Sephacryl S-200 gel 
      filtration column to separate alpha1-antichymotrypsin-complexed PSA (ACT-PSA) and 
      free PSA. Values of ACT-PSA decreased with increasing incubation temperature and 
      time, whereas free-PSA remained relatively constant. The optimal temperature and 
      time for incubation were 58 degrees C and 30 min. Using free/total-PSA ratios, we 
      were able to distinguish between benign prostatic hyperplasia and prostate 
      carcinoma in patients whose PSA was in the diagnostic 'grey zone', i.e. 4.1 to 
      10.0 ng/ml. Through receiver operating characteristic curve analysis, the area 
      under the curve increased from 0.675 to 0.871 when comparing the performance of 
      total PSA to the free/total-PSA ratio. Thus, clinical application of our present 
      methodology may reduce the need to obtain prostatic biopsies in patients whose 
      PSA level is within the diagnostic 'grey zone'.
FAU - Yoshida, K
AU  - Yoshida K
AD  - Department of Urology, Dokkyo University School of Medicine, Tochigi, Japan.
FAU - Honda, M
AU  - Honda M
FAU - Sumi, S
AU  - Sumi S
FAU - Arai, K
AU  - Arai K
FAU - Suzuki, S
AU  - Suzuki S
FAU - Kitahara, S
AU  - Kitahara S
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Clin Chim Acta
JT  - Clinica chimica acta; international journal of clinical chemistry
JID - 1302422
RN  - EC 3.4.21.77 (Prostate-Specific Antigen)
SB  - IM
MH  - Chromatography, Gel
MH  - Diagnosis, Differential
MH  - Hot Temperature
MH  - Humans
MH  - Male
MH  - Prostate-Specific Antigen/*blood
MH  - Prostatic Hyperplasia/diagnosis/immunology
MH  - Prostatic Neoplasms/*diagnosis/immunology
MH  - ROC Curve
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Specimen Handling/*methods
EDAT- 1999/03/25 00:00
MHDA- 1999/03/25 00:01
CRDT- 1999/03/25 00:00
PHST- 1999/03/25 00:00 [pubmed]
PHST- 1999/03/25 00:01 [medline]
PHST- 1999/03/25 00:00 [entrez]
AID - S0009-8981(98)00189-2 [pii]
AID - 10.1016/s0009-8981(98)00189-2 [doi]
PST - ppublish
SO  - Clin Chim Acta. 1999 Feb;280(1-2):195-203. doi: 10.1016/s0009-8981(98)00189-2.