PMID- 10090587
OWN - NLM
STAT- MEDLINE
DCOM- 19990504
LR  - 20190905
IS  - 0721-832X (Print)
IS  - 0721-832X (Linking)
VI  - 237
IP  - 3
DP  - 1999 Mar
TI  - Human trabecular meshwork cells as a thyroid hormone target tissue: presence of 
      functional thyroid hormone receptors.
PG  - 231-40
AB  - PURPOSE: To determine whether human trabecular meshwork cells (HTM) are a 
      potential target tissue for thyroid hormone (3,3',5-triiodothyronine, T3). 
      METHODS: Cultured HTM were assayed for the presence of thyroid hormone receptors 
      (TRs) and retinoid X receptors (RXRs) by reverse-transcriptase polymerase chain 
      reaction (RT-PCR) to detected TR and RXR mRNA, and by immunohistochemistry to 
      detect nuclear TR and RXR proteins. Functionality of the TR was determined by 
      analysis of 125I-T3 binding affinity and capacity in HTM nuclear extracts. 
      Effects of T3 on modulation of hyaluronic acid (HA) levels in HTM were measured 
      as a function of dose and duration of T3 administration. RESULTS: Analysis of 
      RT-PCR and immunohistochemistry demonstrated that cultured HTM expressed 
      TRalpha1, TRalpha2, and TRbeta1 but not TRbeta2; and RXRalpha but not RXRbeta and 
      RXRgamma isoforms. Saturation binding and analysis of 125I-T3 to HTM nuclear 
      extracts revealed Kd of 57 pM. The number of T3 binding sites extrapolated from a 
      Scatchard plot was 7.3 x 10(10)/microg of HTM nuclear protein extract. T3 
      supplementation reduced the concentration of HA in the cell medium by 32-43% 
      compared to cells grown in the absence of T3. CONCLUSIONS: Cultured HTM express 
      three TR isoforms and one RXR isoform, bind T3 with an affinity similar to that 
      of TR in responsive cells, and modulate their HA production in response to T3. 
      These findings indicate that the human trabecular meshwork tissue has the 
      capacity to respond to thyroid hormones.
FAU - Duncan, K G
AU  - Duncan KG
AD  - Department of Ophthalmology, University of California, San Francisco 94143, USA.
FAU - Jumper, M D
AU  - Jumper MD
FAU - Ribeiro, R C
AU  - Ribeiro RC
FAU - Bailey, K R
AU  - Bailey KR
FAU - Yen, P M
AU  - Yen PM
FAU - Sugawara, A
AU  - Sugawara A
FAU - Patel, A
AU  - Patel A
FAU - Stern, R
AU  - Stern R
FAU - Chin, W W
AU  - Chin WW
FAU - Baxter, J D
AU  - Baxter JD
FAU - Schwartz, D M
AU  - Schwartz DM
LA  - eng
GR  - EY02162/EY/NEI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Germany
TA  - Graefes Arch Clin Exp Ophthalmol
JT  - Graefe's archive for clinical and experimental ophthalmology = Albrecht von 
      Graefes Archiv fur klinische und experimentelle Ophthalmologie
JID - 8205248
RN  - 0 (DNA Primers)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Receptors, Thyroid Hormone)
RN  - 0 (Retinoid X Receptors)
RN  - 0 (Transcription Factors)
RN  - 06LU7C9H1V (Triiodothyronine)
SB  - IM
MH  - Adult
MH  - Cell Count/drug effects
MH  - Cells, Cultured/drug effects
MH  - DNA Primers/chemistry
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Nuclear Proteins/drug effects/genetics/metabolism
MH  - RNA, Messenger/analysis
MH  - Receptors, Retinoic Acid/drug effects/genetics/metabolism
MH  - Receptors, Thyroid Hormone/drug effects/genetics/*metabolism
MH  - Retinoid X Receptors
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Trabecular Meshwork/cytology/drug effects/*metabolism
MH  - Transcription Factors/drug effects/genetics/metabolism
MH  - Triiodothyronine/pharmacology
EDAT- 1999/03/25 00:00
MHDA- 1999/03/25 00:01
CRDT- 1999/03/25 00:00
PHST- 1999/03/25 00:00 [pubmed]
PHST- 1999/03/25 00:01 [medline]
PHST- 1999/03/25 00:00 [entrez]
AID - 10.1007/s004170050224 [doi]
PST - ppublish
SO  - Graefes Arch Clin Exp Ophthalmol. 1999 Mar;237(3):231-40. doi: 
      10.1007/s004170050224.