PMID- 10092066
OWN - NLM
STAT- MEDLINE
DCOM- 19990413
LR  - 20041117
IS  - 0023-6837 (Print)
IS  - 0023-6837 (Linking)
VI  - 79
IP  - 3
DP  - 1999 Mar
TI  - Nuclear/cytoplasmic localization of the multiple endocrine neoplasia type 1 gene 
      product, menin.
PG  - 301-10
AB  - Although the gene responsible for multiple endocrine neoplasia, type 1 (MEN1) has 
      been identified recently, the function of its gene product, menin, is not known. 
      To examine menin's biological role, we created an N-terminal tagged fusion 
      protein to follow the distribution of menin in the cell. In all cell lines 
      tested, menin was found both in the nucleus and the cytoplasm, but its 
      localization was dependent on the phase of the cell cycle; during a nondividing 
      phase, menin was found in the nucleus; during and immediately after cell 
      division, it was found in the cytoplasm. To confirm the cellular localization 
      seen with the N-terminal tagged protein, we developed and purified 
      peptide-specific antibodies. One of these antibodies (NCI 624), which recognizes 
      a domain (aa 383-395) of menin, was used in immunofluorescence studies to 
      corroborate the N-terminal tagging results. Further confirmation of menin 
      localization was obtained in a pituitary tumor cell line derived from a familial 
      MEN1 patient, which contained a mixed cell population with either none, or one 
      functional copy of the MEN1 gene. Our results indicate that menin functions 
      principally as a nuclear protein but may be found in the cytoplasm during cell 
      division.
FAU - Huang, S C
AU  - Huang SC
AD  - Laboratory of Pathology, National Cancer Institute, National Institutes of 
      Health, Bethesda, Maryland 20892-1500, USA.
FAU - Zhuang, Z
AU  - Zhuang Z
FAU - Weil, R J
AU  - Weil RJ
FAU - Pack, S
AU  - Pack S
FAU - Wang, C
AU  - Wang C
FAU - Krutzsch, H C
AU  - Krutzsch HC
FAU - Pham, T A
AU  - Pham TA
FAU - Lubensky, I A
AU  - Lubensky IA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Lab Invest
JT  - Laboratory investigation; a journal of technical methods and pathology
JID - 0376617
RN  - 0 (MEN1 protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proto-Oncogene Proteins)
SB  - IM
MH  - Amino Acid Sequence/genetics
MH  - Cell Nucleus/*metabolism
MH  - Cytoplasm/*metabolism
MH  - Fluorescent Antibody Technique
MH  - HeLa Cells
MH  - Humans
MH  - Molecular Sequence Data
MH  - Neoplasm Proteins/*metabolism
MH  - Pituitary Neoplasms/metabolism
MH  - *Proto-Oncogene Proteins
MH  - Sequence Tagged Sites
MH  - Subcellular Fractions/metabolism
MH  - Tissue Distribution/physiology
MH  - Tumor Cells, Cultured
EDAT- 1999/03/26 00:00
MHDA- 1999/03/26 00:01
CRDT- 1999/03/26 00:00
PHST- 1999/03/26 00:00 [pubmed]
PHST- 1999/03/26 00:01 [medline]
PHST- 1999/03/26 00:00 [entrez]
PST - ppublish
SO  - Lab Invest. 1999 Mar;79(3):301-10.