PMID- 10092147
OWN - NLM
STAT- MEDLINE
DCOM- 19990511
LR  - 20190915
IS  - 0300-5623 (Print)
IS  - 0300-5623 (Linking)
VI  - 27
IP  - 1
DP  - 1999
TI  - Characterization of chromosome 8 aberrations in the prostate cancer cell line 
      LNCaP-FGC and sublines.
PG  - 3-8
AB  - In two androgen-dependent (FGC and P70) and two androgen-independent (LNO and R) 
      sublines of the prostate cancer model LNCaP numerical and structural aberrations 
      of chromosome 8 were investigated in detail. The techniques used were whole 
      chromosome paint (WCP) and fluorescence in situ hybridization (FISH) with three 
      cosmid probes mapping to different parts of the p-arm (D8S7 (8p23.3), LPL (8p22) 
      and PLAT (8p11.1)). By WCP all four cell lines showed four copies of chromosome 8 
      in most cells. However, FISH demonstrated that in all sublines deletions in the 
      8p region were present. The majority of both FGC and P70 had two copies of 
      cosmids D8S7 and LPL. The cosmid PLAT showed a broader distribution (1-4 copies), 
      especially in P70. Compared with FGC and P70, both LNO and R showed a larger 
      number of copies (3 or 4) of all three cosmid loci. It is discussed that this 
      difference is probably the result of nondisjunction as a reaction to loss of 
      other sequences on 8p, possibly the tumor suppressor gene (TSG) mapping to 8p21. 
      The fact that both sublines LNO and R are androgen-independent raises the 
      possibility of a link between TSG loss on 8p and androgen independence.
FAU - Konig, J J
AU  - Konig JJ
AD  - Department of Urology, Erasmus University Rotterdam, The Netherlands.
FAU - Teubel, W
AU  - Teubel W
FAU - van Steenbrugge, G J
AU  - van Steenbrugge GJ
FAU - Romijn, J C
AU  - Romijn JC
FAU - Hagemeijer, A
AU  - Hagemeijer A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - Germany
TA  - Urol Res
JT  - Urological research
JID - 0364311
SB  - IM
MH  - Chromosome Aberrations/*genetics
MH  - Chromosomes, Human, Pair 8/*genetics
MH  - Cosmids/genetics
MH  - Gene Deletion
MH  - Gene Dosage
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Molecular Biology/methods
MH  - Prostatic Neoplasms/*genetics
MH  - Tumor Cells, Cultured
RF  - 19
EDAT- 1999/03/26 00:00
MHDA- 1999/03/26 00:01
CRDT- 1999/03/26 00:00
PHST- 1999/03/26 00:00 [pubmed]
PHST- 1999/03/26 00:01 [medline]
PHST- 1999/03/26 00:00 [entrez]
AID - 10.1007/s002400050082 [doi]
PST - ppublish
SO  - Urol Res. 1999;27(1):3-8. doi: 10.1007/s002400050082.