PMID- 10092655
OWN - NLM
STAT- MEDLINE
DCOM- 19990427
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 14
DP  - 1999 Apr 2
TI  - Effect of cold shock on lipid A biosynthesis in Escherichia coli. Induction At 12 
      degrees C of an acyltransferase specific for palmitoleoyl-acyl carrier protein.
PG  - 9677-85
AB  - Palmitoleate is not present in lipid A isolated from Escherichia coli grown at 30 
      degrees C or higher, but it comprises approximately 11% of the fatty acyl chains 
      of lipid A in cells grown at 12 degrees C. The appearance of palmitoleate at 12 
      degrees C is accompanied by a decline in laurate from approximately 18% to 
      approximately 5.5%. We now report that wild-type E. coli shifted from 30 degrees 
      C to 12 degrees C acquire a novel palmitoleoyl-acyl carrier protein 
      (ACP)-dependent acyltransferase that acts on the key lipid A precursor Kdo2-lipid 
      IVA. The palmitoleoyl transferase is induced more than 30-fold upon cold shock, 
      as judged by assaying extracts of cells shifted to 12 degrees C. The induced 
      activity is maximal after 2 h of cold shock, and then gradually declines but does 
      not disappear. Strains harboring an insertion mutation in the lpxL(htrB) gene, 
      which encodes the enzyme that normally transfers laurate from lauroyl-ACP to 
      Kdo2-lipid IVA (Clementz, T., Bednarski, J. J., and Raetz, C. R. H. (1996) J. 
      Biol. Chem. 271, 12095-12102) are not defective in the cold-induced palmitoleoyl 
      transferase. Recently, a gene displaying 54% identity and 73% similarity at the 
      protein level to lpxL was found in the genome of E. coli. This lpxL homologue, 
      designated lpxP, encodes the cold shock-induced palmitoleoyl transferase. 
      Extracts of cells containing lpxP on the multicopy plasmid pSK57 exhibit a 
      10-fold increase in the specific activity of the cold-induced palmitoleoyl 
      transferase compared with cells lacking the plasmid. The elevated specific 
      activity of the palmitoleoyl transferase under conditions of cold shock is 
      attributed to greatly increased levels of lpxP mRNA. The replacement of laurate 
      with palmitoleate in lipid A may reflect the desirability of maintaining the 
      optimal outer membrane fluidity at 12 degrees C.
FAU - Carty, S M
AU  - Carty SM
AD  - Department of Biochemistry, Duke University Medical Center, Durham, North 
      Carolina 27710, USA.
FAU - Sreekumar, K R
AU  - Sreekumar KR
FAU - Raetz, C R
AU  - Raetz CR
LA  - eng
SI  - GENBANK/U49787
GR  - GM-34123/GM/NIGMS NIH HHS/United States
GR  - GM-51310/GM/NIGMS NIH HHS/United States
GR  - GM08558/GM/NIGMS NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Acyl Carrier Protein)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Fatty Acids, Monounsaturated)
RN  - 0 (Lipid A)
RN  - 0 (RNA, Messenger)
RN  - 143600-83-3 (3-deoxy-2-octulosonic acid(2)-lipid IV(A))
RN  - 209B6YPZ4I (palmitoleic acid)
RN  - EC 2.3.- (Acyltransferases)
RN  - EC 2.3.- (LpxL protein, E coli)
RN  - EC 2.3.- (LpxL protein, bacteria)
RN  - EC 2.3.1.- (lpxP protein, E coli)
SB  - IM
MH  - Acyl Carrier Protein/*metabolism
MH  - Acylation
MH  - Acyltransferases/*biosynthesis/chemistry/genetics
MH  - Amino Acid Sequence
MH  - *Bacterial Proteins
MH  - Carbohydrate Sequence
MH  - *Cold Temperature
MH  - Enzyme Induction
MH  - Escherichia coli/*metabolism
MH  - *Escherichia coli Proteins
MH  - Fatty Acids, Monounsaturated/metabolism
MH  - Lipid A/analogs & derivatives/*biosynthesis/metabolism
MH  - Models, Chemical
MH  - Molecular Sequence Data
MH  - RNA, Messenger/metabolism
MH  - Salmonella typhimurium
EDAT- 1999/03/27 00:00
MHDA- 1999/03/27 00:01
CRDT- 1999/03/27 00:00
PHST- 1999/03/27 00:00 [pubmed]
PHST- 1999/03/27 00:01 [medline]
PHST- 1999/03/27 00:00 [entrez]
AID - S0021-9258(19)87304-0 [pii]
AID - 10.1074/jbc.274.14.9677 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Apr 2;274(14):9677-85. doi: 10.1074/jbc.274.14.9677.