PMID- 10096130
OWN - NLM
STAT- MEDLINE
DCOM- 19990525
LR  - 20190921
IS  - 0923-2508 (Print)
IS  - 0923-2508 (Linking)
VI  - 150
IP  - 1
DP  - 1999 Jan-Feb
TI  - Secondary metabolites of Aspergillus exert immunobiological effects on human 
      monocytes.
PG  - 13-9
AB  - This project focused on the effects of aflatoxin B1 (AFB1), a food-contaminating 
      mycotoxin produced by fungi, genus Aspergillus, on the release and genetic 
      expression of some important cytokines, i.e., (interleukin-1 alpha (IL-1 alpha), 
      IL-6, tumor necrosis factor-alpha (TNF alpha)) by human monocytes. Monocytes, 
      preincubated for different time periods with concentrations of AFB1 ranging from 
      0.01 to 1.0 pg/mL, were then activated with bacterial lipopolysaccharide. 
      Cytokine levels were measured by immunoassay and mRNA by cDNA amplification. 
      Pretreatment of monocytes with AFB1 resulted in a decrease in IL-1, IL-6 and TNF 
      alpha release already at a concentration of 0.05 pg/mL. The gene expression of 
      the cytokines considered was drastically affected by treatment with AFB1. In 
      fact, AFB1 completely blocked the transcription of IL-1 alpha, IL-6 and TNF alpha 
      mRNAs, while it did not affect beta-actin mRNA at the concentrations used. It 
      therefore appears that AFB1 exerts its effect on cytokine release through 
      selective inhibition of specific mRNA, without affecting general protein 
      synthesis.
FAU - Rossano, F
AU  - Rossano F
AD  - Institute of Microbiology, Medical School, 2nd University of Naples, Italy. 
      rossano@unina.it
FAU - Ortega De Luna, L
AU  - Ortega De Luna L
FAU - Buommino, E
AU  - Buommino E
FAU - Cusumano, V
AU  - Cusumano V
FAU - Losi, E
AU  - Losi E
FAU - Catania, M R
AU  - Catania MR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Res Microbiol
JT  - Research in microbiology
JID - 8907468
RN  - 0 (Carcinogens)
RN  - 0 (Cytokines)
RN  - 9N2N2Y55MH (Aflatoxin B1)
SB  - IM
MH  - Aflatoxin B1/*toxicity
MH  - Aspergillus/*metabolism
MH  - Carcinogens/*toxicity
MH  - Cytokines/biosynthesis/*drug effects
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - Leukocytes, Mononuclear/*drug effects/immunology
MH  - Time Factors
EDAT- 1999/03/30 00:00
MHDA- 1999/03/30 00:01
CRDT- 1999/03/30 00:00
PHST- 1999/03/30 00:00 [pubmed]
PHST- 1999/03/30 00:01 [medline]
PHST- 1999/03/30 00:00 [entrez]
AID - S0923250899800424 [pii]
AID - 10.1016/s0923-2508(99)80042-4 [doi]
PST - ppublish
SO  - Res Microbiol. 1999 Jan-Feb;150(1):13-9. doi: 10.1016/s0923-2508(99)80042-4.