PMID- 10098017
OWN - NLM
STAT- MEDLINE
DCOM- 19990524
LR  - 20081121
IS  - 0021-9975 (Print)
IS  - 0021-9975 (Linking)
VI  - 120
IP  - 1
DP  - 1999 Jan
TI  - Distribution of a Korean strain of porcine reproductive and respiratory syndrome 
      virus in experimentally infected pigs, as demonstrated immunohistochemically and 
      by in-situ hybridization.
PG  - 79-88
AB  - In an experiment with 40 specific pathogen-free pigs aged 3 days, the 
      distribution of a Korean isolate of porcine reproductive and respiratory syndrome 
      virus (PRRSV) was assessed immunohistochemically and by in-situ hybridization for 
      a period of 28 days after intranasal inoculation. The most consistent and intense 
      labelling for PRRSV was in the lung, the virus persisting in pulmonary 
      macrophages for at least 28 days. The middle lobe of the lung was the optimum 
      site for the detection of PRRSV antigens and nucleic acids, and the interstitial 
      macrophage was the main cell type in which PRRSV was identified. Other tissues 
      and cells in which the virus was detected included macrophages and dendritic 
      cells in the tonsil, lymph nodes, spleen and Peyer's patches, and macrophages in 
      the hepatic sinusoids and adrenal gland. The experiment suggested that the 
      pathogenesis of PRRSV infection may be summarized thus: initial entry of virus 
      through tonsillar and pulmonary macrophages, followed within 3 days by viraemia 
      and subsequent interstitial pneumonia.
FAU - Cheon, D S
AU  - Cheon DS
AD  - Department of Veterinary Pathology, College of Veterinary Medicine, Seoul 
      National University, Suwon, Kyounggi-Do, Republic of Korea.
FAU - Chae, C
AU  - Chae C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Comp Pathol
JT  - Journal of comparative pathology
JID - 0102444
RN  - 0 (Antigens, Viral)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Antigens, Viral/analysis
MH  - Dendritic Cells/virology
MH  - Immunohistochemistry
MH  - In Situ Hybridization/veterinary
MH  - Lung/pathology/virology
MH  - Lymph Nodes/virology
MH  - Macrophages, Alveolar/virology
MH  - Palatine Tonsil/virology
MH  - Porcine Reproductive and Respiratory Syndrome/pathology/*virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/genetics/immunology/*isolation & purification
MH  - RNA, Viral/analysis
MH  - Specific Pathogen-Free Organisms
MH  - Spleen/virology
MH  - Swine
MH  - Time Factors
EDAT- 1999/03/31 00:00
MHDA- 1999/03/31 00:01
CRDT- 1999/03/31 00:00
PHST- 1999/03/31 00:00 [pubmed]
PHST- 1999/03/31 00:01 [medline]
PHST- 1999/03/31 00:00 [entrez]
AID - S0021-9975(98)90257-2 [pii]
AID - 10.1053/jcpa.1998.0257 [doi]
PST - ppublish
SO  - J Comp Pathol. 1999 Jan;120(1):79-88. doi: 10.1053/jcpa.1998.0257.