PMID- 10099442
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 1097-0290 (Electronic)
IS  - 0006-3592 (Linking)
VI  - 60
IP  - 3
DP  - 1998 Nov 5
TI  - Characterization of fluid-flow resistance in root cultures with a convective flow 
      tubular bioreactor.
PG  - 375-84
AB  - Agrobacterium transformed root cultures of Hyoscyamus muticus were grown in a 
      recirculating 2 L tubular bioreactor system. Performance of this convective flow 
      reactor (CFR) was compared to a bubble column (BC) reactor of the same geometry: 
      replicated CFR experiments produced an average tissue concentration of 556 +/- 4 
      grams fresh weight per liter in 30 d whereas the bubble column produced only 328 
      +/- 5 grams per liter corresponding to 25.3 +/- 0.0 and 14.3 +/- 0.5 grams dry 
      weight per liter, respectively. Because media nutrient levels were maintained 
      sufficiently high to saturate growth rate, the improved performance of the CFR is 
      attributed to enhanced convective mass transfer. The pressure drops observed for 
      flow through roots grown within the reactors were more than an order of magnitude 
      higher than previously obtained by placing roots grown in shake culture into 
      defined geometries. The experimentally observed flow resistance was much higher 
      than would be predicted from correlations using the root diameter as the 
      characteristic diameter for flow resistance. Several lines of evidence suggest 
      that root hairs are a substantial contributor to the observed high flow 
      resistance in these transformed root cultures. Pressure drop increased 
      nonlinearly with velocity which could not be adequately described by a modified 
      form of the Ergun equation. Kyan et al's (1970) equation, although predicting 
      such curvature, relies almost exclusively on an empirical packing deflection term 
      to describe the hydrodynamic behavior. Implications of these results to the 
      design of submerged reactor systems for root culture are discussed. Copyright 
      1998 John Wiley & Sons, Inc.
FAU - Carvalho, EB
AU  - Carvalho EB
AD  - Department of Chemical Engineering, 108 Fenske Laboratory, The Pennsylvania State 
      University, University Park, Pennsylvania 16802, USA.
FAU - Curtis, WR
AU  - Curtis WR
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biotechnol Bioeng
JT  - Biotechnology and bioengineering
JID - 7502021
EDAT- 1999/04/01 03:08
MHDA- 1999/04/01 03:09
CRDT- 1999/04/01 03:08
PHST- 1999/04/01 03:08 [pubmed]
PHST- 1999/04/01 03:09 [medline]
PHST- 1999/04/01 03:08 [entrez]
AID - 10.1002/(SICI)1097-0290(19981105)60:3<375::AID-BIT15>3.0.CO;2-L [pii]
AID - 10.1002/(sici)1097-0290(19981105)60:3<375::aid-bit15>3.0.co;2-l [doi]
PST - ppublish
SO  - Biotechnol Bioeng. 1998 Nov 5;60(3):375-84. doi: 
      10.1002/(sici)1097-0290(19981105)60:3<375::aid-bit15>3.0.co;2-l.