PMID- 10099875 OWN - NLM STAT- MEDLINE DCOM- 19990706 LR - 20191103 IS - 0967-3849 (Print) IS - 0967-3849 (Linking) VI - 6 IP - 8 DP - 1998 Dec TI - Polymorphism of the nucleolus organizer regions (NORs) in Physalaemus petersi (Amphibia, Anura, Leptodactylidae) detected by silver staining and fluorescence in situ hybridization. PG - 621-8 AB - The nucleolus organizer regions (NORs) of both karyotypes I and II of Physalaemus petersi (Jimenez de la Espada, 1872) from the Brazilian Amazon were studied by Giemsa staining, and by the Ag-NOR method. Karyological group I specimens were also studied by the fluorescence in situ hybridization (FISH) technique. Multiple NOR-bearing chromosomes were detected in both karyotypes. The coincident results of the Ag-NOR and FISH methods rule out the occurrence of silent NORs in this anuran. There was no intraindividual NOR variability in either group, but interindividual variability of NORs was high in group I. Seven different patterns of active NOR distribution were definitely recognized among fifteen specimens. This was considered to be a NOR site polymorphism. These results, combined with the C-band polymorphism previously reported for P. petersi, demonstrate a high rate of chromosome evolution in this group. FAU - Lourenco, L B AU - Lourenco LB AD - Department of Cell Biology, Biology Institute, Campinas, SP, Brazil. FAU - Recco-Pimentel, S M AU - Recco-Pimentel SM FAU - Cardoso, A J AU - Cardoso AJ LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Netherlands TA - Chromosome Res JT - Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology JID - 9313452 SB - IM MH - Animals MH - Anura/*genetics MH - Chromosomes/genetics MH - Female MH - *In Situ Hybridization, Fluorescence MH - Interphase MH - Karyotyping MH - Male MH - Metaphase MH - Nucleolus Organizer Region/*genetics/ultrastructure MH - Polymorphism, Genetic/*genetics MH - *Silver Staining EDAT- 1999/04/01 00:00 MHDA- 1999/04/01 00:01 CRDT- 1999/04/01 00:00 PHST- 1999/04/01 00:00 [pubmed] PHST- 1999/04/01 00:01 [medline] PHST- 1999/04/01 00:00 [entrez] AID - 10.1023/a:1009253410553 [doi] PST - ppublish SO - Chromosome Res. 1998 Dec;6(8):621-8. doi: 10.1023/a:1009253410553.