PMID- 10100736
OWN - NLM
STAT- MEDLINE
DCOM- 19990517
LR  - 20190921
IS  - 0742-3098 (Print)
IS  - 0742-3098 (Linking)
VI  - 26
IP  - 2
DP  - 1999 Mar
TI  - Protective effect of melatonin on indomethacin-induced gastric injury in rats.
PG  - 101-7
AB  - The gastric injury associated with nonsteroidal anti-inflammatory drug (NSAID) 
      therapy has been linked to the detrimental effects of the agents on the processes 
      of prostaglandin synthesis, neutrophil (PMN) activation. and oxygen free radical 
      generation. In the present study, we investigated the in vivo protective effects 
      of melatonin on indomethacin-induced gastric lesions in the rat. Peroxidation of 
      lipids and changes in the activities of related enzymes such as glutathione 
      peroxidase (GSH-px) and myeloperoxidase (MPO), as a marker of PMNs infiltration, 
      were also studied. Intraperitoneal (i.p.) injection of melatonin (0.25. 0.5, 1 mg 
      kg(-1)) 30 min before indomethacin administration prevented gastric injury. The 
      mean ulcer indices significantly (P < 0.05) decreased. Thiobarbituric acid (TBA) 
      reactive substances in the gastric mucosa as an index of peroxidation, was 
      increased after indomethacin administration and this increase was inhibited by 
      melatonin. In addition, pretreatment with melatonin resulted in a significant 
      increase of the enzymatic GSH-px activity up to the control levels; however, 
      inhibition of ulceration by melatonin was not associated with a significant 
      reduction in PMN infiltration. These results suggest that the protection afforded 
      by the pineal hormone against indomethacin-induced gastric injury may be, in 
      addition to other possible mechanisms, to its radical scavenging activity.
FAU - Alarcon de la Lastra, C
AU  - Alarcon de la Lastra C
AD  - Laboratorio de Farmacologia, Facultad de Farmacia, Universidad de Sevilla, Spain. 
      Alarcon@fafar.us.es
FAU - Motilva, V
AU  - Motilva V
FAU - Martin, M J
AU  - Martin MJ
FAU - Nieto, A
AU  - Nieto A
FAU - Barranco, M D
AU  - Barranco MD
FAU - Cabeza, J
AU  - Cabeza J
FAU - Herrerias, J M
AU  - Herrerias JM
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Pineal Res
JT  - Journal of pineal research
JID - 8504412
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - JL5DK93RCL (Melatonin)
RN  - XXE1CET956 (Indomethacin)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents, Non-Steroidal/*toxicity
MH  - Female
MH  - Free Radical Scavengers
MH  - Gastric Mucosa/metabolism/pathology
MH  - Glutathione Peroxidase/metabolism
MH  - Indomethacin/*toxicity
MH  - Lipid Peroxidation
MH  - Male
MH  - Melatonin/*therapeutic use
MH  - Neutrophils/pathology
MH  - Peroxidase/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Stomach Ulcer/chemically induced/*prevention & control
MH  - Thiobarbituric Acid Reactive Substances/metabolism
EDAT- 1999/04/01 00:00
MHDA- 1999/04/01 00:01
CRDT- 1999/04/01 00:00
PHST- 1999/04/01 00:00 [pubmed]
PHST- 1999/04/01 00:01 [medline]
PHST- 1999/04/01 00:00 [entrez]
AID - 10.1111/j.1600-079x.1999.tb00569.x [doi]
PST - ppublish
SO  - J Pineal Res. 1999 Mar;26(2):101-7. doi: 10.1111/j.1600-079x.1999.tb00569.x.