PMID- 10101239
OWN - NLM
STAT- MEDLINE
DCOM- 19990521
LR  - 20190826
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 67
IP  - 1
DP  - 1999 Apr 6
TI  - Hippocampal neurotrophin and trk receptor mRNA levels are altered by local 
      administration of nicotine, carbachol and pilocarpine.
PG  - 124-36
AB  - Cholinergic receptor agonists nicotine (nicotinic), carbachol 
      (nicotinic/muscarinic) and pilocarpine (muscarinic) were administered into the 
      hippocampus and mRNA levels of neurotrophins and their receptors determined using 
      in situ hybridisation. Drug doses were carefully chosen to avoid the potentially 
      confounding effects of seizure and cell death. Nicotine caused a long-lasting 
      increase in nerve growth factor (NGF) mRNA in all subfields of the hippocampus. 
      The increase was evident from 24 h up to 72 h after drug administration. This 
      increase was dependent on excitatory amino acid neurotransmission as it was 
      blocked by administration of an AMPA or NMDA receptor antagonist. In contrast, 
      carbachol and pilocarpine produced a transient increase in NGF mRNA levels 
      present 4-8 h after drug administration. Pilocarpine caused a transient increase 
      in hippocampal brain-derived neurotrophic factor (BDNF) levels, with carbachol 
      and nicotine showing the same trend. Nicotine and carbachol caused transient 
      decreases in NT-3 mRNA levels in dentate gyrus and CA2 with pilocarpine showing a 
      similar trend. Increases in mRNA encoding full-length trkB were seen 8 h after 
      nicotine, with nicotine also causing elevations in a mRNA encoding a truncated 
      isoform (trkB.T2). TrkC mRNA was not altered by any of the conditions used. The 
      study suggests that muscarinic and nicotinic receptor activation in the 
      hippocampus causes transient changes in all of the neurotrophins, but that NGF 
      levels are selectively up-regulated by nicotinic receptor stimulation. The 
      reciprocal interaction between NGF and ascending cholinergic systems may be a 
      component of the cognitive enhancing effects of nicotine.
CI  - Copyright 1999 Elsevier Science B.V.
FAU - French, S J
AU  - French SJ
AD  - Biochemical Neuropharmacology Group, Neuroscience Research Centre and Division of 
      Biochemistry and Molecular Biology, GKT School of Biomedical Sciences, Guy's 
      Hospital Campus, London SE1 9RT, UK.
FAU - Humby, T
AU  - Humby T
FAU - Horner, C H
AU  - Horner CH
FAU - Sofroniew, M V
AU  - Sofroniew MV
FAU - Rattray, M
AU  - Rattray M
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cholinergic Agonists)
RN  - 0 (Muscarinic Agonists)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Neurotrophin 3)
RN  - 0 (Nicotinic Agonists)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptor, Ciliary Neurotrophic Factor)
RN  - 0 (Receptor, Nerve Growth Factor)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - 01MI4Q9DI3 (Pilocarpine)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - 6M3C89ZY6R (Nicotine)
RN  - 8Y164V895Y (Carbachol)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Receptor, trkA)
RN  - EC 2.7.10.1 (Receptor, trkC)
RN  - N9YNS0M02X (Acetylcholine)
SB  - IM
MH  - Acetylcholine/physiology
MH  - Animals
MH  - Behavior, Animal/physiology
MH  - Brain Chemistry/drug effects/physiology
MH  - Brain-Derived Neurotrophic Factor/genetics
MH  - Carbachol/*pharmacology
MH  - Cell Death/drug effects
MH  - Cholinergic Agonists/pharmacology
MH  - Cholinergic Fibers/chemistry/physiology
MH  - Epilepsy/chemically induced/physiopathology
MH  - Gene Expression/drug effects/physiology
MH  - Glutamic Acid/metabolism
MH  - Hippocampus/chemistry/physiology
MH  - In Situ Hybridization
MH  - Male
MH  - Muscarinic Agonists/*pharmacology
MH  - Nerve Growth Factors/*genetics
MH  - Neuroprotective Agents/metabolism
MH  - Neurotrophin 3
MH  - Nicotine/*pharmacology
MH  - Nicotinic Agonists/*pharmacology
MH  - Pilocarpine/*pharmacology
MH  - Proto-Oncogene Proteins/*genetics
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor Protein-Tyrosine Kinases/*genetics
MH  - Receptor, Ciliary Neurotrophic Factor
MH  - Receptor, Nerve Growth Factor
MH  - Receptor, trkA
MH  - Receptor, trkC
MH  - Receptors, Nerve Growth Factor/*genetics
MH  - Septal Nuclei/chemistry/cytology/physiology
MH  - Synaptic Transmission/physiology
EDAT- 1999/04/02 00:00
MHDA- 1999/04/02 00:01
CRDT- 1999/04/02 00:00
PHST- 1999/04/02 00:00 [pubmed]
PHST- 1999/04/02 00:01 [medline]
PHST- 1999/04/02 00:00 [entrez]
AID - S0169328X99000480 [pii]
AID - 10.1016/s0169-328x(99)00048-0 [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 1999 Apr 6;67(1):124-36. doi: 
      10.1016/s0169-328x(99)00048-0.