PMID- 10101240
OWN - NLM
STAT- MEDLINE
DCOM- 19990521
LR  - 20190826
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 67
IP  - 1
DP  - 1999 Apr 6
TI  - Transcriptional regulation of GABAA receptor gamma2 subunit gene.
PG  - 137-47
AB  - We have cloned the promoter regions of the genes for the mouse and human gamma2 
      subunits of the type A receptors for gamma-aminobutyric acid (GABA). For the 
      mouse, the two major transcription start sites were at +1 (by definition) and 
      +43, as established by rapid amplification of cDNA ends (RACE) and primer 
      extension. This numbering places the start methionine at +297. There was no TATA 
      or CCAAT box. Both mouse and human sequences have a candidate neuron-restrictive 
      silencer element (NRSE) site in the first intron (+956 in mouse). We made 
      assorted mouse-based promoter/reporter (luciferase) constructs starting from a 
      core extending from -331 to +136, varying sizes at both ends, and including and 
      excluding the putative NRSE and more proximal sequences. These were tested by 
      transient transfection in several neuron-like and non-neuronal cell lines. Both 
      proximal and distal downstream elements appeared to help direct expression to 
      neuron-like cells, the NRSE in the intron, by repression in non-neurons, and a 
      24-bp portion of the 5' untranslated region starting at +113 (named GPE1) by 
      preferentially promoting expression in neuron-like cells. Cotransfected human 
      NRSF (transcription factor for NRSE) reduced reporter expression in neuron-like 
      cells for constructs containing the NRSE in two locations. In gel mobility shift 
      assays, the mouse gamma2 NRSE and a consensus NRSE both bound in vitro translated 
      NRSF very similarly, and the NRSF gave the same major shifted band with the mouse 
      gamma2 NRSE as was observed with nuclear extracts.
CI  - Copyright 1999 Elsevier Science B.V.
FAU - Mu, W
AU  - Mu W
AD  - Department of Pharmacology and Experimental Therapeutics, University of Maryland 
      School of Medicine, 655 W. Baltimore St., Baltimore, MD 21201-1559, USA.
FAU - Burt, D R
AU  - Burt DR
LA  - eng
GR  - HD16596/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (Receptors, GABA-A)
RN  - 12794-10-4 (Benzodiazepines)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Base Sequence
MH  - Benzodiazepines/pharmacology
MH  - Brain Chemistry/*genetics
MH  - Cloning, Molecular/methods
MH  - DNA Primers
MH  - DNA, Complementary
MH  - Gene Expression Regulation/physiology
MH  - Genes, Reporter
MH  - HeLa Cells
MH  - Humans
MH  - Luciferases/genetics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - Plasmids
MH  - Promoter Regions, Genetic/physiology
MH  - Protein Structure, Tertiary
MH  - Receptors, GABA-A/chemistry/*genetics
MH  - Sequence Homology, Amino Acid
MH  - Transcriptional Activation/drug effects/*physiology
MH  - Transfection
MH  - gamma-Aminobutyric Acid/physiology
EDAT- 1999/04/02 00:00
MHDA- 1999/04/02 00:01
CRDT- 1999/04/02 00:00
PHST- 1999/04/02 00:00 [pubmed]
PHST- 1999/04/02 00:01 [medline]
PHST- 1999/04/02 00:00 [entrez]
AID - S0169328X99000492 [pii]
AID - 10.1016/s0169-328x(99)00049-2 [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 1999 Apr 6;67(1):137-47. doi: 
      10.1016/s0169-328x(99)00049-2.