PMID- 10101598
OWN - NLM
STAT- MEDLINE
DCOM- 19990504
LR  - 20191103
IS  - 0361-090X (Print)
IS  - 0361-090X (Linking)
VI  - 23
IP  - 2
DP  - 1999
TI  - Biomarkers in monitoring for efficacy of immunotherapy and chemoprevention of 
      bladder cancer with dimethylsulfoxide.
PG  - 163-71
AB  - This study correlated biomarkers expressed in tumor and epithelial field with 
      clinical response and recurrence. Of 25 bladder cancer patients, 11 received 6 
      weeks of intravesical Bacille Calmette-Guerin (BCG), and 14 were treated weekly 
      with intravesical dimethylsulfoxide (DMSO) for 4 weeks to further modulate 
      biomarker expression. G-actin, DNA aneuploidy, and p300 tumor antigen were 
      evaluated by quantitative fluorescence image analysis on uroepithelial cells from 
      bladder wash samples prior to and immediately following treatment. Excluding 
      patients who did not respond to BCG (and who had persistently abnormal p300 and 
      DNA markers), recurrence correlated with persistent abnormal G-actin findings. Of 
      patients who were G-actin negative following therapy, only 25% recurred during 
      follow-up in contrast to 67% in patients who were positive (p < 0.03 by Fisher's 
      exact test). The odds ratio for recurrence was 6.00 (95% confidence interval: 
      1.3-28.6). Cytosolic G-actin levels can be an important intermediate end point 
      marker for chemoprevention.
FAU - Hemstreet, G P 3rd
AU  - Hemstreet GP 3rd
AD  - Department of Urology, University of Oklahoma Health Sciences Center, Oklahoma 
      City, USA.
FAU - Rao, J
AU  - Rao J
FAU - Hurst, R E
AU  - Hurst RE
FAU - Bonner, R B
AU  - Bonner RB
FAU - Mellott, J E
AU  - Mellott JE
FAU - Rooker, G M
AU  - Rooker GM
LA  - eng
GR  - R01-DK45368/DK/NIDDK NIH HHS/United States
GR  - R01-OH02647/OH/NIOSH CDC HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Cancer Detect Prev
JT  - Cancer detection and prevention
JID - 7704778
RN  - 0 (Actins)
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (BCG Vaccine)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Cryoprotective Agents)
RN  - 0 (DNA, Neoplasm)
RN  - YOW8V9698H (Dimethyl Sulfoxide)
SB  - IM
MH  - Actins/metabolism
MH  - Adult
MH  - Aged
MH  - Antigens, Neoplasm/metabolism
MH  - BCG Vaccine/*therapeutic use
MH  - Biomarkers, Tumor/*metabolism
MH  - Chemoprevention
MH  - Combined Modality Therapy
MH  - Cryoprotective Agents/*therapeutic use
MH  - DNA, Neoplasm/analysis
MH  - Dimethyl Sulfoxide/*therapeutic use
MH  - Female
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - Immunotherapy, Active
MH  - Male
MH  - Microscopy, Fluorescence
MH  - Middle Aged
MH  - Urinary Bladder Neoplasms/metabolism/*pathology/prevention & control/*therapy
EDAT- 1999/04/02 03:02
MHDA- 2001/03/28 10:01
CRDT- 1999/04/02 03:02
PHST- 1999/04/02 03:02 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1999/04/02 03:02 [entrez]
AID - 10.1046/j.1525-1500.1999.09917.x [doi]
PST - ppublish
SO  - Cancer Detect Prev. 1999;23(2):163-71. doi: 10.1046/j.1525-1500.1999.09917.x.