PMID- 10102086
OWN - NLM
STAT- MEDLINE
DCOM- 19990702
LR  - 20190921
IS  - 1046-7408 (Print)
IS  - 1046-7408 (Linking)
VI  - 41
IP  - 2
DP  - 1999 Feb
TI  - The expression of human leukocyte antigen-G on trophoblasts abolishes the 
      growth-suppressing effect of interleukin-2 towards them.
PG  - 153-8
AB  - PROBLEM: We have shown the attenuated human leukocyte antigen (HLA)-G expression 
      on trophoblasts and an aberrant expression of interleukin (IL)-2, a cytotoxic 
      cytokine, in decidual tissue in preeclampsia, where deteriorated trophoblastic 
      invasion into decidual layers may constitute a crucial pathogenesis. We 
      hypothesized that the absence of HLA-G might make trophoblasts susceptible to 
      compromise by IL-2. METHOD OF STUDY: We analyzed the growth of HLA-G-negative and 
      positive cell lines, all of which possessed IL-2 receptors, in the culture with 
      or without IL-2 supplementation. RESULTS: The proliferation of HLA-G positive 
      trophoblastic cell lines (BeWo and JEG-3) was not influenced by the addition of 
      IL-2, whereas a HLA-G-negative trophoblastic cell line (JAR) exhibited 
      significantly decreased proliferation when cultured with IL-2. Interestingly, the 
      transfection of JAR cells with HLA-G completely eliminates the growth-inhibitory 
      effect of IL-2. CONCLUSION: The expression of HLA-G may commit trophoblasts to 
      evade cell damage by IL-2, which may be relevant to maternal tolerance of the 
      fetus during pregnancy and its derangement as exemplified by preeclampsia.
FAU - Hamai, Y
AU  - Hamai Y
AD  - Department of Obstetrics and Gynecology, Faculty of Medicine, University of 
      Tokyo, Japan.
FAU - Fujii, T
AU  - Fujii T
FAU - Yamashita, T
AU  - Yamashita T
FAU - Miki, A
AU  - Miki A
FAU - Hyodo, H
AU  - Hyodo H
FAU - Kozuma, S
AU  - Kozuma S
FAU - Geraghty, D E
AU  - Geraghty DE
FAU - Taketani, Y
AU  - Taketani Y
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Interleukin-2)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Interleukin-2)
SB  - IM
CIN - Am J Reprod Immunol. 1999 Feb;41(2):117-20. PMID: 10102083
MH  - Cell Division/*drug effects
MH  - Cell Line
MH  - Flow Cytometry
MH  - HLA Antigens/*physiology
MH  - HLA-G Antigens
MH  - Histocompatibility Antigens Class I/*physiology
MH  - Humans
MH  - Interleukin-2/metabolism/*pharmacology
MH  - Leukocytes, Mononuclear
MH  - RNA, Messenger/genetics/metabolism
MH  - Receptors, Interleukin-2/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Trophoblasts/*cytology/*immunology
MH  - Tumor Cells, Cultured
EDAT- 1999/04/02 00:00
MHDA- 1999/04/02 00:01
CRDT- 1999/04/02 00:00
PHST- 1999/04/02 00:00 [pubmed]
PHST- 1999/04/02 00:01 [medline]
PHST- 1999/04/02 00:00 [entrez]
AID - 10.1111/j.1600-0897.1999.tb00088.x [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 1999 Feb;41(2):153-8. doi: 
      10.1111/j.1600-0897.1999.tb00088.x.