PMID- 10102087
OWN - NLM
STAT- MEDLINE
DCOM- 19990702
LR  - 20190921
IS  - 1046-7408 (Print)
IS  - 1046-7408 (Linking)
VI  - 41
IP  - 2
DP  - 1999 Feb
TI  - Analysis of human leukocyte antigen-G polymorphism including intron 4 in Japanese 
      couples with habitual abortion.
PG  - 159-63
AB  - PROBLEM: The purpose of this study was to clarify whether there is a difference 
      between the allele frequency of human leukocyte antigen (HLA)-G in healthy 
      Japanese people and that of Japanese couples with habitual abortion. METHOD OF 
      STUDY: Exons 2, 3, 4, and intron 4 of the HLA-G gene were analyzed in 20 couples 
      with habitual abortion, using polymerase chain reaction (PCR)-single-strand 
      conformation polymorphism (SSCP) analysis. Intron 4 of the HLA-G gene was also 
      analyzed in 54 healthy individuals. The nucleotide sequence of the PCR product of 
      intron 4 was further determined by direct sequencing. RESULTS: Two kinds of 
      nucleotide sequence were identified in intron 4 of the HLA-G gene, one of which 
      was identical to that of HLA-G*01011, and the other was identical to that of 
      HLA-G*01012, G*01013, and G*0104. The frequency of each allele in affected women 
      and their husbands did not significantly differ from that of healthy individuals, 
      and no mutation was found in any affected couple. CONCLUSION: HLA-G allelic 
      abnormality seemed to have little, if any, implication in the pathogenesis of 
      habitual abortion.
FAU - Yamashita, T
AU  - Yamashita T
AD  - Department of Obstetrics and Gynecology, Faculty of Medicine, University of 
      Tokyo, Japan.
FAU - Fujii, T
AU  - Fujii T
FAU - Tokunaga, K
AU  - Tokunaga K
FAU - Tadokoro, K
AU  - Tadokoro K
FAU - Hamai, Y
AU  - Hamai Y
FAU - Miki, A
AU  - Miki A
FAU - Kozuma, S
AU  - Kozuma S
FAU - Juji, T
AU  - Juji T
FAU - Taketani, Y
AU  - Taketani Y
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
CIN - Am J Reprod Immunol. 1999 Feb;41(2):117-20. PMID: 10102083
MH  - Abortion, Habitual/*genetics
MH  - Alleles
MH  - Base Sequence
MH  - Exons/genetics
MH  - Female
MH  - HLA Antigens/*genetics
MH  - HLA-G Antigens
MH  - Histocompatibility Antigens Class I/*genetics
MH  - Humans
MH  - Introns/*genetics
MH  - Japan
MH  - Male
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction/methods
MH  - *Polymorphism, Genetic
MH  - Polymorphism, Single-Stranded Conformational
MH  - Pregnancy
MH  - Sequence Analysis, DNA
EDAT- 1999/04/02 00:00
MHDA- 1999/04/02 00:01
CRDT- 1999/04/02 00:00
PHST- 1999/04/02 00:00 [pubmed]
PHST- 1999/04/02 00:01 [medline]
PHST- 1999/04/02 00:00 [entrez]
AID - 10.1111/j.1600-0897.1999.tb00089.x [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 1999 Feb;41(2):159-63. doi: 
      10.1111/j.1600-0897.1999.tb00089.x.