PMID- 10102624
OWN - NLM
STAT- MEDLINE
DCOM- 19990422
LR  - 20071114
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 18
IP  - 8
DP  - 1999 Feb 25
TI  - Suppression of tumorigenicity in human ovarian cancer cell lines is controlled by 
      a 2 cM fragment in chromosomal region 6q24-q25.
PG  - 1545-51
AB  - Multiple distinct regions of chromosome 6 are frequently affected by losses of 
      heterozygosity in primary human ovarian carcinomas. We introduced a normal human 
      chromosome 6 into HEY and SKOV-3 ovarian carcinoma cell lines using 
      microcell-mediated chromosome transfer techniques to further investigate the role 
      of this chromosome in ovarian tumorigenesis. The exogenous chromosome was stably 
      propagated in the recipient cells based on fluorescence in situ hybridization 
      (FISH) analyses with a chromosome 6 painting probe. The tumorigenicity of HEY and 
      SKOV-3 cells was completely suppressed after transfer of chromosome 6, but not 
      after transfer of a chromosome 11q13-qter fragment used as control. Using 46 
      polymorphic microsatellite markers, the region bounded by D6S1649 and D6S1564 was 
      found to be commonly deleted in HEY: chromosome 6 tumorigenic revertant clones. 
      The boundaries of the commonly deleted region could be further narrowed down to a 
      2 cM (based on the Whitehead genetic map) or 0.36 megabase (based on gdb mapping 
      data) region between D6S1637 and D6S1564 after transferring the exogenous 
      chromosome from revertants into mouse L cells and performing allelic deletion 
      mapping studies against this mouse background. We conclude that this region 
      contains a tumor suppressor gene important for the control of ovarian tumor 
      development.
FAU - Wan, M
AU  - Wan M
AD  - Department of Pathology, USC/Norris Comprehensive Cancer Center, USC School of 
      Medicine, Los Angeles, California 90033, USA.
FAU - Sun, T
AU  - Sun T
FAU - Vyas, R
AU  - Vyas R
FAU - Zheng, J
AU  - Zheng J
FAU - Granada, E
AU  - Granada E
FAU - Dubeau, L
AU  - Dubeau L
LA  - eng
GR  - R01 CA51167/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
SB  - IM
MH  - Animals
MH  - Chromosome Painting
MH  - Chromosomes, Human, Pair 6/*genetics
MH  - Female
MH  - Gene Transfer Techniques
MH  - Humans
MH  - Hybrid Cells
MH  - Mice
MH  - Mice, Nude
MH  - Neoplasm Transplantation
MH  - Ovarian Neoplasms/genetics/*pathology
MH  - Physical Chromosome Mapping
MH  - Tumor Cells, Cultured
EDAT- 1999/04/02 00:00
MHDA- 1999/04/02 00:01
CRDT- 1999/04/02 00:00
PHST- 1999/04/02 00:00 [pubmed]
PHST- 1999/04/02 00:01 [medline]
PHST- 1999/04/02 00:00 [entrez]
AID - 10.1038/sj.onc.1202476 [doi]
PST - ppublish
SO  - Oncogene. 1999 Feb 25;18(8):1545-51. doi: 10.1038/sj.onc.1202476.