PMID- 10103023
OWN - NLM
STAT- MEDLINE
DCOM- 19990506
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 260
IP  - 3
DP  - 1999 Mar
TI  - Expression of 3beta-hydroxysteroid dehydrogenase type I and type VI isoforms in 
      the mouse testis during development.
PG  - 911-7
AB  - Six isoforms of the enzyme 3beta-hydroxysteroid dehydrogenase (3betaHSD) have 
      been identified in the mouse, each the product of a distinct gene. Two of these 
      isoforms (type I and type VI) are detectable in the adult testis but changes in 
      their expression during development are unknown. In this study we have examined 
      changes in testicular expression and localization of mRNA encoding the type I and 
      type VI isoforms of 3betaHSD. Total 3betaHSD (type I plus type VI) mRNA was 
      measured by reverse transcription-polymerase chain reaction and showed a peak of 
      expression at day 5 after birth followed by a decline and then a further rise 
      after day 10 that continued up to adulthood. When each isoform was measured 
      individually it was clear that the type I isoform was expressed at all ages from 
      embryonic day 13 to adulthood. In contrast, the type VI isoform was only 
      expressed at significant levels during fetal life on embryonic day 13 and then 
      not again until after day 10 postnatally. Expression of the type VI isoform mRNA 
      increased markedly after day 10 so that by adulthood it was the predominant 
      3betaHSD isoform present in the testis. Closer examination of the timing of type 
      VI expression showed that the isoform mRNA was first detectable at a significant 
      level on day 11. In-situ hybridization confirmed that the type I isoform is the 
      only one expressed in the fetal/neonatal animal and showed that expression was 
      limited to the interstitial tissue. In the adult, both type I and type VI 
      expression was within the interstitial tissue. The timing of 3betaHSD type VI 
      mRNA expression suggests, strongly, that this isoform is expressed only by 
      adult-type Leydig cells in the mouse testis and that this development starts 
      shortly before day 11. The limited expression of the type VI isoform means that 
      it will be a useful marker in studies of adult Leydig cell development.
FAU - Baker, P J
AU  - Baker PJ
AD  - Division of Veterinary Physiology, Department of Veterinary Pre-clinical Studies, 
      University of Glasgow Veterinary School, UK.
FAU - Sha, J A
AU  - Sha JA
FAU - McBride, M W
AU  - McBride MW
FAU - Peng, L
AU  - Peng L
FAU - Payne, A H
AU  - Payne AH
FAU - O'Shaughnessy, P J
AU  - O'Shaughnessy PJ
LA  - eng
GR  - U54 HD31398/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (Isoenzymes)
RN  - 0 (RNA, Messenger)
RN  - EC 1.1.- (3-Hydroxysteroid Dehydrogenases)
SB  - IM
MH  - 3-Hydroxysteroid Dehydrogenases/*biosynthesis/genetics
MH  - Animals
MH  - Female
MH  - In Situ Hybridization
MH  - In Vitro Techniques
MH  - Isoenzymes/*biosynthesis/genetics
MH  - Leydig Cells/enzymology
MH  - Male
MH  - Mice
MH  - RNA, Messenger/biosynthesis
MH  - Testis/cytology/*enzymology/*growth & development
EDAT- 1999/04/02 00:00
MHDA- 1999/04/02 00:01
CRDT- 1999/04/02 00:00
PHST- 1999/04/02 00:00 [pubmed]
PHST- 1999/04/02 00:01 [medline]
PHST- 1999/04/02 00:00 [entrez]
AID - 10.1046/j.1432-1327.1999.00245.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1999 Mar;260(3):911-7. doi: 10.1046/j.1432-1327.1999.00245.x.