PMID- 10103229
OWN - NLM
STAT- MEDLINE
DCOM- 19991005
LR  - 20240322
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 65
IP  - 4
DP  - 1999 Apr
TI  - Initial reactions in the biodegradation of 1-chloro-4-nitrobenzene by a newly 
      isolated bacterium, strain LW1.
PG  - 1405-12
AB  - Bacterial strain LW1, which belongs to the family Comamonadaceae, utilizes 
      1-chloro-4-nitrobenzene (1C4NB) as a sole source of carbon, nitrogen, and energy. 
      Suspensions of 1C4NB-grown cells removed 1C4NB from culture fluids, and there was 
      a concomitant release of ammonia and chloride. Under anaerobic conditions LW1 
      transformed 1C4NB into a product which was identified as 2-amino-5-chlorophenol 
      by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy and mass 
      spectrometry. This transformation indicated that there was partial reduction of 
      the nitro group to the hydroxylamino substituent, followed by Bamberger 
      rearrangement. In the presence of oxygen but in the absence of NAD, fast 
      transformation of 2-amino-5-chlorophenol into a transiently stable yellow product 
      was observed with resting cells and cell extracts. This compound exhibited an 
      absorption maximum at 395 nm and was further converted to a dead-end product with 
      maxima at 226 and 272 nm. The compound formed was subsequently identified by 1H 
      and 13C NMR spectroscopy and mass spectrometry as 5-chloropicolinic acid. In 
      contrast, when NAD was added in the presence of oxygen, only minor amounts of 
      5-chloropicolinic acid were formed, and a new product, which exhibited an 
      absorption maximum at 306 nm, accumulated.
FAU - Katsivela, E
AU  - Katsivela E
AD  - Division of Microbiology, GBF-National Research Centre for Biotechnology, D-38124 
      Braunschweig, Germany. EKA@gbf.de
FAU - Wray, V
AU  - Wray V
FAU - Pieper, D H
AU  - Pieper DH
FAU - Wittich, R M
AU  - Wittich RM
LA  - eng
SI  - GENBANK/AJ130765
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Chlorophenols)
RN  - 0 (Culture Media)
RN  - 0 (Nitrobenzenes)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 28443-50-7 (2-amino-5-chlorophenol)
RN  - CVL66U249D (4-chloronitrobenzene)
RN  - EC 1.13.- (Oxygenases)
SB  - IM
MH  - Aerobiosis
MH  - Anaerobiosis
MH  - Bacteria/classification/*growth & development/*metabolism
MH  - Biodegradation, Environmental
MH  - Chlorophenols/metabolism
MH  - Culture Media
MH  - Magnetic Resonance Spectroscopy
MH  - Molecular Sequence Data
MH  - Nitrobenzenes/*metabolism
MH  - Oxygenases/metabolism
MH  - RNA, Ribosomal, 16S/genetics
PMC - PMC91199
EDAT- 1999/04/02 00:00
MHDA- 1999/04/02 00:01
PMCR- 1999/04/01
CRDT- 1999/04/02 00:00
PHST- 1999/04/02 00:00 [pubmed]
PHST- 1999/04/02 00:01 [medline]
PHST- 1999/04/02 00:00 [entrez]
PHST- 1999/04/01 00:00 [pmc-release]
AID - 1427 [pii]
AID - 10.1128/AEM.65.4.1405-1412.1999 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 1999 Apr;65(4):1405-12. doi: 
      10.1128/AEM.65.4.1405-1412.1999.