PMID- 10148311
OWN - NLM
STAT- MEDLINE
DCOM- 19930527
LR  - 20041117
IS  - 0955-3886 (Print)
IS  - 0955-3886 (Linking)
VI  - 14
IP  - 1
DP  - 1993 Jan
TI  - Leukocyte-depleted platelets prepared from pooled buffy coat post-transfusion 
      increment and "in vitro bleeding time" using the Thrombostat 4000/2.
PG  - 35-9
AB  - A technique for preparation of platelet concentrate (PC) from pooled buffy coat 
      (BC) is described in this study. The yield of platelets from 4 BCs was 
      310+/-48x10 9 per unit with a leukocyte content of 21+/-20x10 6 per unit. When a 
      leukocyte removing filter was interconnected, Sepacell 5N and Pall PL-50 filters, 
      no leukocytes could be found in 56 of 59 and in 36 of 37 PCs, with a maximum 
      content of 2.3 and 0.54x10 6 leukocytes per PC, respectively. The platelet yield 
      was 276+/-37 and 279+/-54x10 9 per unit, respectively. The in vitro bleeding time 
      (IVBT), using the Thrombostat 4000/2, corresponded with the corrected count 
      increment in most of 11 thrombocytopenic patients receiving platelet 
      transfusions. However, in two transfusions no correction of IVBT was obtained. 
      This technique offers a new possibility to investigate the functional capacity of 
      transfused platelets.
FAU - Hogman, C F
AU  - Hogman CF
AD  - Department of Clinical Immunology and Transfusion Medicine, University Hospital, 
      Uppsala, Sweden.
FAU - Eriksson, L
AU  - Eriksson L
FAU - Kristensen, J
AU  - Kristensen J
LA  - eng
PT  - Journal Article
PL  - England
TA  - Transfus Sci
JT  - Transfusion science
JID - 9001514
MH  - *Bleeding Time
MH  - Blood Component Removal/*instrumentation/methods
MH  - Blood Component Transfusion/instrumentation/methods
MH  - *Blood Platelets
MH  - Filtration
MH  - Humans
MH  - Leukocyte Count/instrumentation/methods
MH  - Platelet Count/instrumentation/methods
EDAT- 1992/12/09 00:00
MHDA- 1992/12/09 00:01
CRDT- 1992/12/09 00:00
PHST- 1992/12/09 00:00 [pubmed]
PHST- 1992/12/09 00:01 [medline]
PHST- 1992/12/09 00:00 [entrez]
AID - 0955-3886(93)90051-U [pii]
AID - 10.1016/0955-3886(93)90051-U [doi]
PST - ppublish
SO  - Transfus Sci. 1993 Jan;14(1):35-9. doi: 10.1016/0955-3886(93)90051-U.