PMID- 10148312
OWN - NLM
STAT- MEDLINE
DCOM- 19930527
LR  - 20071115
IS  - 0955-3886 (Print)
IS  - 0955-3886 (Linking)
VI  - 14
IP  - 1
DP  - 1993 Jan
TI  - Platelet concentrates from buffy coats: improved conditions for preparation and 
      evaluation in routine clinical use.
PG  - 41-6
AB  - Since 1990, platelet concentrates prepared by soft centrifugation of buffy-coat 
      pools diluted with a glucose-free, commercially available crystalloid solution 
      (BC-PC) are the first choice product for all platelet recipients in our 
      institution. Numerous in vitro and in vivo observations from our own and other 
      laboratories indicate that BC-PC compare favorably to PC prepared from 
      platelet-rich plasma (PRP). In the present in vitro study we evaluated 
      traditional and bottom-and-top bags and modified centrifugation conditions with 
      the aim of increasing in vitro platelet yield in BC-PC. This was 14-18% higher 
      compared with our previous protocol when prolonged centrifugation and 
      bottom-and-top bags were used. In addition, we evaluated post-transfusion 
      platelet count increments in 42 unselected adult hematological patients routinely 
      transfused with 703 1-5 day-old BC-PC pools. Transfusion data were managed with 
      PLATELET, an MS-DOS compatible program which includes automated calculation of 
      transfusion efficacy and periodic patient reports. Mean pre-, 1 h and 24 h 
      post-transfusion platelet counts were 16, 38 and 28x10 9/L, respectively. Mean 1 
      h and 24 h post-transfusion platelet count increments, expressed as percentage of 
      expected, were 40 and 24%, respectively. These data were similar to those 
      obtained previously in 189 unselected hematological patients given 2432 PRP-PC 
      transfusions (mean 1 h post-transfusion increment 46% of expected). The present 
      in vitro study confirms that similar platelet yields can be obtained with the BC 
      and PRP methods. In vivo findings show that also in routine conditions 
      post-transfusion increments of PRP-PC and BC-PC are similar.(ABSTRACT TRUNCATED 
      AT 250 WORDS)
FAU - Rebulla, P
AU  - Rebulla P
AD  - Centro Trasfusionale e di Immunologia dei Trapianti, Ospedale Maggiore, Milano, 
      Italy.
FAU - Bertolini, F
AU  - Bertolini F
FAU - Porretti, L
AU  - Porretti L
FAU - Marangoni, F
AU  - Marangoni F
FAU - Smacchia, C
AU  - Smacchia C
FAU - Marconi, M
AU  - Marconi M
FAU - Riccardi, D
AU  - Riccardi D
FAU - Sirelson, V
AU  - Sirelson V
FAU - Pappalettera, M
AU  - Pappalettera M
FAU - Sirchia, G
AU  - Sirchia G
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Transfus Sci
JT  - Transfusion science
JID - 9001514
MH  - Adult
MH  - Blood Component Removal/*methods
MH  - Blood Component Transfusion/*methods
MH  - *Blood Platelets
MH  - Evaluation Studies as Topic
MH  - Humans
MH  - Platelet Count/methods
MH  - *Software
EDAT- 1992/12/09 00:00
MHDA- 1992/12/09 00:01
CRDT- 1992/12/09 00:00
PHST- 1992/12/09 00:00 [pubmed]
PHST- 1992/12/09 00:01 [medline]
PHST- 1992/12/09 00:00 [entrez]
AID - 0955-3886(93)90052-V [pii]
AID - 10.1016/0955-3886(93)90052-V [doi]
PST - ppublish
SO  - Transfus Sci. 1993 Jan;14(1):41-6. doi: 10.1016/0955-3886(93)90052-V.