PMID- 10149417
OWN - NLM
STAT- MEDLINE
DCOM- 19920827
LR  - 20041117
IS  - 0894-959X (Print)
IS  - 0894-959X (Linking)
VI  - 4
IP  - 6
DP  - 1991 Nov-Dec
TI  - Isolation of large numbers of fully viable human reticulocytes using continuous 
      Percoll density gradient.
PG  - 360-4
AB  - A rapid one-step continuous density gradient centrifugation method for the 
      separation of reticulocytes from peripheral blood is described. Each of 27 blood 
      samples from patients whose initial reticulocytes ranged from 2.1% to 22.8% (X = 
      7.6%) was subjected to Percoll density gradient separation. Reticulocyte-rich 
      areas were found in the 1.081 to 1.092 density range. The final reticulocyte 
      yield ranged from 73.9% to 98.6% (X = 87.0%) and a recovery of 14.8%. Optimal 
      reticulocyte yields were obtained using centrifugation tubes with an internal 
      diameter of 16 mm and when the tubes were spun for 40 minutes. Two indices that 
      may be reliable indicators of final reticulocyte yield were the mean corpuscular 
      hemoglobin concentration (MCHC). This simple and reliable method can be used to 
      separate platelets, granulocytes, erythrocytes, and mononuclear cells.
FAU - Pawar, V B
AU  - Pawar VB
AD  - Department of Clinical Laboratory Sciences, University of Nevada-Las Vegas 89154.
FAU - Prabhu, A
AU  - Prabhu A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Clin Lab Sci
JT  - Clinical laboratory science : journal of the American Society for Medical 
      Technology
JID - 8806547
MH  - Centrifugation, Density Gradient/*methods
MH  - Erythrocyte Count
MH  - Humans
MH  - Regression Analysis
MH  - *Reticulocytes
EDAT- 1991/10/06 00:00
MHDA- 1991/10/06 00:01
CRDT- 1991/10/06 00:00
PHST- 1991/10/06 00:00 [pubmed]
PHST- 1991/10/06 00:01 [medline]
PHST- 1991/10/06 00:00 [entrez]
PST - ppublish
SO  - Clin Lab Sci. 1991 Nov-Dec;4(6):360-4.