PMID- 10188273
OWN - NLM
STAT- MEDLINE
DCOM- 19990414
LR  - 20061115
IS  - 0723-2020 (Print)
IS  - 0723-2020 (Linking)
VI  - 22
IP  - 1
DP  - 1999 Feb
TI  - In situ detection of Escherichia coli cells containing ColE1-related plasmids by 
      hybridization to regulatory RNA II.
PG  - 1-8
AB  - A method is described for the in situ detection of individual whole fixed cells 
      of Escherichia coli containing ColE1-related plasmids. It makes use of 
      fluorescence in situ hybridization (FISH) and the regulatory RNA II as a target 
      molecule for both, Cy3- and HRP-labeled olinucleotide probes. Various methods for 
      signal amplification were compared. Probes targeting the regulatory RNA I did not 
      result in the in situ detection of plasmid-bearing cells.
FAU - Juretschko, S
AU  - Juretschko S
AD  - Lehrstuhl fur Mikrobiologie, Technische Universitat Munchen, Germany.
FAU - Schonhuber, W
AU  - Schonhuber W
FAU - Kulakauskas, S
AU  - Kulakauskas S
FAU - Ehrlich, D S
AU  - Ehrlich DS
FAU - Schleifer, K H
AU  - Schleifer KH
FAU - Amann, R
AU  - Amann R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Syst Appl Microbiol
JT  - Systematic and applied microbiology
JID - 8306133
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA primers)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Escherichia coli/genetics/*isolation & purification
MH  - *In Situ Hybridization, Fluorescence
MH  - Oligonucleotide Probes
MH  - *Plasmids
MH  - RNA/*genetics
EDAT- 1999/04/03 00:00
MHDA- 1999/04/03 00:01
CRDT- 1999/04/03 00:00
PHST- 1999/04/03 00:00 [pubmed]
PHST- 1999/04/03 00:01 [medline]
PHST- 1999/04/03 00:00 [entrez]
AID - S0723-2020(99)80022-8 [pii]
AID - 10.1016/S0723-2020(99)80022-8 [doi]
PST - ppublish
SO  - Syst Appl Microbiol. 1999 Feb;22(1):1-8. doi: 10.1016/S0723-2020(99)80022-8.