PMID- 10189364
OWN - NLM
STAT- MEDLINE
DCOM- 19990506
LR  - 20190508
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 145
IP  - 1
DP  - 1999 Apr 5
TI  - Evidence for a relatively random array of human chromosomes on the mitotic ring.
PG  - 1-14
AB  - We used fluorescence in situ hybridization (FISH) to study the positions of human 
      chromosomes on the mitotic rings of cultured human lymphocytes, MRC-5 
      fibroblasts, and CCD-34Lu fibroblasts. The homologous chromosomes of all three 
      cell types had relatively random positions with respect to each other on the 
      mitotic rings of prometaphase rosettes and anaphase cells. Also, the positions of 
      the X and Y chromosomes, colocalized with the somatic homologues in male cells, 
      were highly variable from one mitotic ring to another. Although random 
      chromosomal positions were found in different pairs of CCD-34Lu and MRC-5 
      late-anaphases, the separations between the same homologous chromosomes in paired 
      late-anaphase and telophase chromosomal masses were highly correlated. Thus, 
      although some loose spatial associations of chromosomes secondary to interphase 
      positioning may exist on the mitotic rings of some cells, a fixed order of human 
      chromosomes and/or a rigorous separation of homologous chromosomes on the mitotic 
      ring are not necessary for normal mitosis. Furthermore, the relative chromosomal 
      positions on each individual metaphase plate are most likely carried through 
      anaphase into telophase.
FAU - Allison, D C
AU  - Allison DC
AD  - Department of Surgery, MCO Microscopy Imaging Center, Medical College of Ohio, 
      Toledo, Ohio 43614, USA. dallison@mco.edu
FAU - Nestor, A L
AU  - Nestor AL
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
SB  - IM
MH  - Anaphase
MH  - Cells, Cultured
MH  - Chromosomes, Human/*ultrastructure
MH  - Female
MH  - Fibroblasts/*ultrastructure
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphocytes/*ultrastructure
MH  - Male
MH  - Metaphase
MH  - *Mitosis
MH  - Models, Genetic
MH  - Telophase
PMC - PMC2148215
EDAT- 1999/04/06 00:00
MHDA- 1999/04/06 00:01
PMCR- 1999/10/05
CRDT- 1999/04/06 00:00
PHST- 1999/04/06 00:00 [pubmed]
PHST- 1999/04/06 00:01 [medline]
PHST- 1999/04/06 00:00 [entrez]
PHST- 1999/10/05 00:00 [pmc-release]
AID - 10.1083/jcb.145.1.1 [doi]
PST - ppublish
SO  - J Cell Biol. 1999 Apr 5;145(1):1-14. doi: 10.1083/jcb.145.1.1.