PMID- 10189943
OWN - NLM
STAT- MEDLINE
DCOM- 19990511
LR  - 20141120
IS  - 0951-418X (Print)
IS  - 0951-418X (Linking)
VI  - 13
IP  - 1
DP  - 1999 Feb
TI  - Effects of the aqueous fraction of the ethanol extract of the leaves of 
      Cissampelos sympodialis Eichl. in human neutrophils.
PG  - 9-13
AB  - An aqueous fraction (10-300 micrograms/mL) of the ethanol extract of the leaves 
      of Cissampelos sympodialis Eichl inhibited N-formyl-Met-Leu-Phe (fMLP)-induced 
      release of lysozyme and myeloperoxidase from human neutrophils. Inhibition by the 
      fraction, as well as by dibutyryl-cAMP and prostaglandin E2, was substantially 
      greater when the cells were pretreated with the phosphodiesterase (PDE) inhibitor 
      isobutyl methyl xanthine (IBMX) indicating that the effect may be mediated by 
      cAMP. Measurement of intracellular cAMP levels showed that the fraction (30-100 
      micrograms/mL) increased the nucleotide levels in IBMX-pretreated neutrophils 
      which was unaffected by propranolol. Cyclic AMP dependent protein kinase A 
      activity was also increased by the fraction (1.5-100 micrograms/mL). Superoxide 
      anion generation induced by fMLP in cytochalasin B-treated cells primed with PAF 
      was not inhibited by the aqueous fraction. The results indicate that the aqueous 
      fraction of Cissampelos sympodialis inhibits neutrophil degranulation by a 
      cAMP-dependent mechanism which may be relevant to the use of the plant as an 
      anti-asthmatic agent in folk medicine.
FAU - Thomas, G
AU  - Thomas G
AD  - Laboratorio de Tecnologia Farmaceutica, Universidade Federal da Paraiba, Joao 
      Pessoa, PB, Brazil.
FAU - Selak, M
AU  - Selak M
FAU - Henson, P M
AU  - Henson PM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Phytother Res
JT  - Phytotherapy research : PTR
JID - 8904486
RN  - 0 (Plant Extracts)
RN  - 0 (Solvents)
RN  - 059QF0KO0R (Water)
RN  - 11062-77-4 (Superoxides)
RN  - 3K9958V90M (Ethanol)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - EC 3.2.1.17 (Muramidase)
SB  - IM
MH  - Brazil
MH  - Cyclic AMP/metabolism
MH  - Cyclic AMP-Dependent Protein Kinases/metabolism
MH  - Ethanol
MH  - Humans
MH  - In Vitro Techniques
MH  - Muramidase/metabolism
MH  - Neutrophils/*drug effects/enzymology/metabolism
MH  - Peroxidase/metabolism
MH  - Plant Extracts/*pharmacology
MH  - Plant Leaves/chemistry
MH  - Plants, Medicinal/*chemistry
MH  - Solvents
MH  - Superoxides/metabolism
MH  - Water
EDAT- 1999/04/06 00:00
MHDA- 1999/04/06 00:01
CRDT- 1999/04/06 00:00
PHST- 1999/04/06 00:00 [pubmed]
PHST- 1999/04/06 00:01 [medline]
PHST- 1999/04/06 00:00 [entrez]
AID - 10.1002/(SICI)1099-1573(199902)13:1<9::AID-PTR389>3.0.CO;2-E [doi]
PST - ppublish
SO  - Phytother Res. 1999 Feb;13(1):9-13. doi: 
      10.1002/(SICI)1099-1573(199902)13:1<9::AID-PTR389>3.0.CO;2-E.