PMID- 10190553
OWN - NLM
STAT- MEDLINE
DCOM- 19990413
LR  - 20231213
IS  - 0143-3334 (Print)
IS  - 0143-3334 (Linking)
VI  - 20
IP  - 3
DP  - 1999 Mar
TI  - Regulation of connexin32 and connexin43 gene expression by DNA methylation in rat 
      liver cells.
PG  - 401-6
AB  - Gap junction proteins (connexins) are expressed in a cell-specific manner and 
      expression is often reduced in neoplastic cells. We investigated the mechanisms 
      of connexin32 (Cx32) and connexin43 (Cx43) expression in hepatic cells using 
      MH1C1 rat hepatoma cells and freshly isolated, adult rat hepatocytes that express 
      Cx32 but not Cx43 and WB-F344 rat liver epithelial cells that express Cx43 but 
      not Cx32. Southern blotting after DNA restriction with MspI and HpaII indicated 
      that two MspI/HpaII restriction sites in the Cx32 promoter (positions -147 and 
      -847) were methylated in WB-F344 cells, but not in MH1C1 cells or hepatocytes. In 
      contrast, an MspI/HpaII restriction site in the Cx43 promoter (position -38) was 
      methylated in MH1C1 cells, but not in WB-F344 cells or hepatocytes. Transient 
      transfection of the cell lines with connexin promoter-luciferase constructs 
      indicated that the Cx32 promoter was 7-fold more active in MH1C1 cells and the 
      Cx43 promoter was 5-fold more active in WB-F344 cells. These results suggest that 
      transcription of Cx32 and Cx43 in hepatic cells is controlled by promoter 
      methylation and by cell-specific transcription factors. Similar mechanisms may be 
      involved in the reduced expression of these genes frequently observed in 
      neoplastic cells.
FAU - Piechocki, M P
AU  - Piechocki MP
AD  - Department of Pathology, Medical College of Ohio, Toledo 43699-0008, USA.
FAU - Burk, R D
AU  - Burk RD
FAU - Ruch, R J
AU  - Ruch RJ
LA  - eng
GR  - CA-R29-57612/CA/NCI NIH HHS/United States
GR  - DK-5P30-41296/DK/NIDDK NIH HHS/United States
GR  - DK-P01-41918/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Carcinogenesis
JT  - Carcinogenesis
JID - 8008055
RN  - 0 (Connexin 43)
RN  - 0 (Connexins)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Connexin 43/*genetics
MH  - Connexins/*genetics
MH  - *DNA Methylation
MH  - Dexamethasone/pharmacology
MH  - Gene Expression Regulation/drug effects/*genetics
MH  - Liver/cytology/drug effects/*metabolism
MH  - Promoter Regions, Genetic
MH  - Rats
MH  - Rats, Inbred F344
MH  - Transfection
MH  - Gap Junction beta-1 Protein
EDAT- 1999/04/06 00:00
MHDA- 1999/04/06 00:01
CRDT- 1999/04/06 00:00
PHST- 1999/04/06 00:00 [pubmed]
PHST- 1999/04/06 00:01 [medline]
PHST- 1999/04/06 00:00 [entrez]
AID - 10.1093/carcin/20.3.401 [doi]
PST - ppublish
SO  - Carcinogenesis. 1999 Mar;20(3):401-6. doi: 10.1093/carcin/20.3.401.