PMID- 10190863
OWN - NLM
STAT- MEDLINE
DCOM- 19990610
LR  - 20190826
IS  - 0304-4017 (Print)
IS  - 0304-4017 (Linking)
VI  - 81
IP  - 3
DP  - 1999 Mar 1
TI  - Isoenzyme activities of different strains of Cryptosporidium parvum.
PG  - 195-200
AB  - Different isoenzyme activities have been assayed in three strains of 
      Cryptosporidium parvum, C1 (C. parvum from infected calves, UK), C2 (C. parvum 
      from infected calves, Egypt) and C3 (C. parvum from infected goats, Egypt). The 
      electrophoretic variations of five enzymes; lactate dehydrogenase (LDH), glucose 
      phosphate isomerase (GPI), hexokinase (HK), malate dehydrogenase (MDH) and 
      glutamate dehydrogenase (GLDH) were compared among the three different isolates 
      using native polyacrylamide gel-electrophoresis. LDH showed an identical pattern 
      in the three isolates. GPI showed two different bands in C3 and C1, with both 
      bands present in C2. HK activity showed a weak band in C1 but no reaction was 
      detected with C2 and C3. Malate dehydrogenase (MDH) showed no reaction in C1, but 
      similar bands in C2 and C3. Glutamate dehydrogenase (GLDH) showed two different 
      patterns, C2 and C3 had one pattern and C1 showed additional zones of reaction. 
      Rat liver homogenate was run at the same time as the parasite extracts as a 
      positive control. This investigation suggests that GPI, HK and GLDH could be used 
      to characterise different Cryptosporidium isolates.
FAU - El-Ghaysh, A
AU  - El-Ghaysh A
AD  - Parasitology Department, Faculty of Veterinary Medicine, Cairo University, Giza, 
      Egypt. elghaysh@hotmail.com
FAU - Barrett, J
AU  - Barrett J
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Vet Parasitol
JT  - Veterinary parasitology
JID - 7602745
RN  - 0 (Isoenzymes)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 1.1.1.37 (Malate Dehydrogenase)
RN  - EC 1.4.1.2 (Glutamate Dehydrogenase)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 5.3.1.9 (Glucose-6-Phosphate Isomerase)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cattle Diseases/*parasitology
MH  - Cryptosporidiosis/parasitology/*veterinary
MH  - Cryptosporidium parvum/*classification/enzymology
MH  - Egypt
MH  - Electrophoresis
MH  - Glucose-6-Phosphate Isomerase/metabolism
MH  - Glutamate Dehydrogenase/metabolism
MH  - Goat Diseases/*parasitology
MH  - Goats
MH  - Hexokinase/metabolism
MH  - Isoenzymes/metabolism
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Malate Dehydrogenase/metabolism
MH  - United Kingdom
EDAT- 1999/04/06 02:03
MHDA- 2000/06/10 09:00
CRDT- 1999/04/06 02:03
PHST- 1999/04/06 02:03 [pubmed]
PHST- 2000/06/10 09:00 [medline]
PHST- 1999/04/06 02:03 [entrez]
AID - S0304-4017(98)00249-0 [pii]
AID - 10.1016/s0304-4017(98)00249-0 [doi]
PST - ppublish
SO  - Vet Parasitol. 1999 Mar 1;81(3):195-200. doi: 10.1016/s0304-4017(98)00249-0.