PMID- 10191987
OWN - NLM
STAT- MEDLINE
DCOM- 19990528
LR  - 20191103
VI  - 15
IP  - 2
DP  - 1999 Apr
TI  - Nucleus extraction from single mounted tissue sections.
PG  - 65-9
AB  - Extraction of nuclei from unmounted archived tissue has become a method widely 
      used for molecular cytogenetic investigations. It is a suitable approach to take 
      advantage of the large series of formalin fixed and subsequently 
      paraffin-embedded material which has been collected in the times before 
      interphase cytogenetic analysis has become possible. We present a new kind of 
      assay for the extraction of nuclei from one single mounted tissue section; the 
      extracted interphase nuclei are suitable very well for a fluorescence in situ 
      hybridization (FISH) approach. The method described has been successfully used 
      for the analysis of the INT2/FGF3-amplicon in 20 samples of oral squamous cell 
      carcinoma.
FAU - Liehr, T
AU  - Liehr T
AD  - Institute of Human Genetics, Jena, Germany. i8lith@mti-n.mti.uni-jena.de
FAU - Uwe, C
AU  - Uwe C
FAU - Erich, G
AU  - Erich G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Genet Anal
JT  - Genetic analysis : biomolecular engineering
JID - 9509403
RN  - 0 (Cell Extracts)
RN  - 0 (FGF3 protein, human)
RN  - 0 (Fibroblast Growth Factor 3)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 62031-54-3 (Fibroblast Growth Factors)
SB  - IM
MH  - Carcinoma, Squamous Cell/genetics/pathology
MH  - Cell Extracts
MH  - Cell Nucleus
MH  - Fibroblast Growth Factor 3
MH  - Fibroblast Growth Factors/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Microtomy
MH  - Mouth Neoplasms/genetics/pathology
MH  - Proto-Oncogene Proteins/genetics
EDAT- 1999/04/07 00:00
MHDA- 1999/04/07 00:01
CRDT- 1999/04/07 00:00
PHST- 1999/04/07 00:00 [pubmed]
PHST- 1999/04/07 00:01 [medline]
PHST- 1999/04/07 00:00 [entrez]
AID - 10.1016/s1050-3862(98)00037-0 [doi]
PST - ppublish
SO  - Genet Anal. 1999 Apr;15(2):65-9. doi: 10.1016/s1050-3862(98)00037-0.