PMID- 10192156
OWN - NLM
STAT- MEDLINE
DCOM- 19990520
LR  - 20190722
IS  - 0011-9059 (Print)
IS  - 0011-9059 (Linking)
VI  - 38
IP  - 2
DP  - 1999 Feb
TI  - Relevance of complement fixing antinuclear antibodies.
PG  - 96-100
AB  - BACKGROUND: Connective tissue diseases (CTDs) are a heterogeneous group of 
      disorders defined by the association of a variety of clinical manifestations with 
      immunologic and other laboratory findings. Overlap of syndromes and aberrant 
      findings appear rather frequently. METHODS: Sera of eight antinuclear antibody 
      (ANA) negative, cases of subacute cutaneous lupus erythematosus (SCLE) with 
      antibodies to Ro (SS-A) and a ninth case with clinical and laboratory signs of 
      Sjogren's syndrome and systemic lupus erythematosus (SLE) were tested for 
      complement (C') fixing antinuclear antibodies (C-ANAs). The ninth case was 
      examined in depth by direct immunofluorescence (DIF) and a two-step "C + DIF" 
      test of biopsies for C' fixation to in vivo bound ANAs, as well as serum tests 
      for C-ANA, ANA, and SCLE markers. RESULTS: Sera of five of the eight ANA 
      negative, Ro(SS-A) positive SCLE cases had C-ANAs. The ninth case, a 50-year-old 
      woman with clinical and laboratory signs of Sjogren's syndrome and SLE, gave a 
      strong positive C + DIF reaction in the skin biopsy for in vivo bound ANAs that 
      fix C', but negative ANAs and C-ANAs in routine serum tests; they revealed 
      antimitochondrial antibodies. Serum tests on normal skin, however, revealed weak 
      ANA and strong C-ANA reactions with in vitro fixed C'. CONCLUSIONS: ANA negative 
      cases of SCLE or Sjogren's syndrome may have C-ANAs. A case with Sjogren's 
      syndrome and signs of SLE had both in vivo and in vitro C' fixing ANAs. C-ANA 
      tests can aid in the identification of such cases.
FAU - Fang, Y V
AU  - Fang YV
AD  - Department of Microbiology, State University of New York at Buffalo, 14214, USA.
FAU - Cyran, S J
AU  - Cyran SJ
FAU - Albini, B
AU  - Albini B
FAU - Rostami, R
AU  - Rostami R
FAU - Binder, W L
AU  - Binder WL
FAU - Beutner, E H
AU  - Beutner EH
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - England
TA  - Int J Dermatol
JT  - International journal of dermatology
JID - 0243704
RN  - 0 (Antibodies, Antinuclear)
RN  - 0 (Biomarkers)
SB  - IM
MH  - Antibodies, Antinuclear/*blood
MH  - Biomarkers/blood
MH  - *Complement Fixation Tests
MH  - Facial Dermatoses/blood/pathology
MH  - Female
MH  - Humans
MH  - Lupus Erythematosus, Systemic/blood/pathology
MH  - Middle Aged
MH  - Sjogren's Syndrome/*blood/pathology
MH  - Skin/chemistry/pathology
EDAT- 1999/04/07 00:00
MHDA- 1999/04/07 00:01
CRDT- 1999/04/07 00:00
PHST- 1999/04/07 00:00 [pubmed]
PHST- 1999/04/07 00:01 [medline]
PHST- 1999/04/07 00:00 [entrez]
AID - 10.1046/j.1365-4362.1999.00580.x [doi]
PST - ppublish
SO  - Int J Dermatol. 1999 Feb;38(2):96-100. doi: 10.1046/j.1365-4362.1999.00580.x.