PMID- 10196153
OWN - NLM
STAT- MEDLINE
DCOM- 19990517
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 16
DP  - 1999 Apr 16
TI  - "Half of the sites" binding of D-glyceraldehyde-3-phosphate dehydrogenase folding 
      intermediate with GroEL.
PG  - 10790-4
AB  - Two D-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) folding intermediate 
      subunits bind with chaperonin 60 (GroEL) to form a stable complex, which can no 
      longer bind with additional GAPDH intermediate subunits, but does bind with one 
      more lysozyme folding intermediate or one chaperonin 10 (GroES) molecule, 
      suggesting that the two GAPDH subunits bind at one end of the GroEL molecule 
      displaying a "half of the sites" binding profile. For lysozyme, GroEL binds with 
      either one or two folding intermediates to form a stable 1:1 or 1:2 complex with 
      one substrate on each end of the GroEL double ring for the latter. The 1:1 
      complex of GroEL.GroES binds with one lysozyme or one dimeric GAPDH folding 
      intermediate to form a stable ternary complex. Both complexes of GroEL.lysozyme1 
      and GroEL.GAPDH2 bind with one GroES molecule only at the other end of the GroEL 
      molecule forming a trans ternary complex. According to the stoichiometry of GroEL 
      binding with the GAPDH folding intermediate and the formation of ternary 
      complexes containing GroEL.GAPDH2, it is suggested that the folding intermediate 
      of GAPDH binds, very likely in the dimeric form, with GroEL at one end only.
FAU - Li, J
AU  - Li J
AD  - National Laboratory of Biomacromolecules, Institute of Biophysics, Academia 
      Sinica, Beijing 100101, China.
FAU - Wang, C C
AU  - Wang CC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Chaperonin 60)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
RN  - EC 3.2.1.17 (Muramidase)
SB  - IM
MH  - Binding Sites
MH  - Chaperonin 60/*metabolism
MH  - Enzyme Activation
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/chemistry/*metabolism
MH  - Muramidase/metabolism
MH  - Oxidation-Reduction
MH  - Protein Denaturation
MH  - Protein Folding
EDAT- 1999/04/10 00:00
MHDA- 1999/04/10 00:01
CRDT- 1999/04/10 00:00
PHST- 1999/04/10 00:00 [pubmed]
PHST- 1999/04/10 00:01 [medline]
PHST- 1999/04/10 00:00 [entrez]
AID - S0021-9258(19)73572-8 [pii]
AID - 10.1074/jbc.274.16.10790 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Apr 16;274(16):10790-4. doi: 10.1074/jbc.274.16.10790.