PMID- 10197789
OWN - NLM
STAT- MEDLINE
DCOM- 19990601
LR  - 20190831
IS  - 0047-6374 (Print)
IS  - 0047-6374 (Linking)
VI  - 107
IP  - 1
DP  - 1999 Feb 1
TI  - Fluorescence in situ hybridization analysis of the fos/jun ratio in the ageing 
      brain.
PG  - 61-71
AB  - We have examined the expression of the fos and jun genes in the cerebellum of the 
      rat brain during ageing, by use of a semi-quantitative fluorescence in situ 
      hybridization (FISH) method. In these experiments we have utilised the digital 
      imaging capabilities of a cooled CCD camera system to store the fluorescence 
      intensities of individual cells and to compare the data from each target (fos or 
      jun) gene with that of a control (beta-actin) gene. In this way we have been able 
      to obtain a relative quantitation of fos and jun mRNA levels. Purkinje cells were 
      analysed in brain from Sprague-Dawley rats of 6, 13 and 23 months of age. Data 
      obtained in this way demonstrated that the level of fos expression decreased 
      significantly during ageing but, in contrast, that of jun increased between 6 and 
      13 months and thereafter remained constant. We subsequently carried out a further 
      comparison of fos/jun ratios in purkinje cells in Wistar rats and also observed a 
      highly significant fall in the ratio between 6 and 23 months. This change in the 
      fos/jun ratio has important implications for the composition of the AP-1 
      transcription factor and for the expression of genes that it regulates.
FAU - Salehi, M
AU  - Salehi M
AD  - School of Health Sciences, University of Sunderland, UK.
FAU - Barron, M
AU  - Barron M
FAU - Merry, B J
AU  - Merry BJ
FAU - Goyns, M H
AU  - Goyns MH
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Mech Ageing Dev
JT  - Mechanisms of ageing and development
JID - 0347227
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Aging/*genetics
MH  - Animals
MH  - Cerebellum/cytology/*metabolism
MH  - Gene Expression
MH  - Genes, fos/genetics
MH  - Genes, jun/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Proto-Oncogene Proteins c-fos/*metabolism
MH  - Proto-Oncogene Proteins c-jun/*metabolism
MH  - Purkinje Cells/cytology/metabolism
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Rats, Wistar
MH  - Reproducibility of Results
MH  - Species Specificity
EDAT- 1999/04/10 00:00
MHDA- 1999/04/10 00:01
CRDT- 1999/04/10 00:00
PHST- 1999/04/10 00:00 [pubmed]
PHST- 1999/04/10 00:01 [medline]
PHST- 1999/04/10 00:00 [entrez]
AID - S0047-6374(98)00137-7 [pii]
AID - 10.1016/s0047-6374(98)00137-7 [doi]
PST - ppublish
SO  - Mech Ageing Dev. 1999 Feb 1;107(1):61-71. doi: 10.1016/s0047-6374(98)00137-7.