PMID- 10198101
OWN - NLM
STAT- MEDLINE
DCOM- 19990524
LR  - 20190514
IS  - 0032-0889 (Print)
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Linking)
VI  - 119
IP  - 4
DP  - 1999 Apr
TI  - Characterization of two divergent endo-beta-1,4-glucanase cDNA clones highly 
      expressed in the nonclimacteric strawberry fruit.
PG  - 1415-22
AB  - Two cDNAs clones (Cel1 and Cel2) encoding divergent endo-beta-1, 4-glucanases 
      (EGases) have been isolated from a cDNA library obtained from ripe strawberry 
      (Fragaria x ananassa Duch) fruit. The analysis of the amino acid sequence 
      suggests that Cel1 and Cel2 EGases have different secondary and tertiary 
      structures and that they differ in the presence of potential N-glycosylation 
      sites. By in vitro translation we show that Cel1 and Cel2 bear a functional 
      signal peptide, the cleavage of which yields mature proteins of 52 and 60 kD, 
      respectively. Phylogenetic analysis revealed that the Cel2 EGase diverged early 
      in evolution from other plant EGases. Northern analysis showed that both EGases 
      are highly expressed in fruit and that they have different temporal patterns of 
      accumulation. The Cel2 EGase was expressed in green fruit, accumulating as the 
      fruit turned from green to white and remaining at an elevated, constant level 
      throughout fruit ripening. In contrast, the Cel1 transcript was not detected in 
      green fruit and only a low level of expression was observed in white fruit. The 
      level of Cel1 mRNA increased gradually during ripening, reaching a maximum in 
      fully ripe fruit. The high levels of Cel1 and Cel2 mRNA in ripe fruit and their 
      overlapping patterns of expression suggest that these EGases play an important 
      role in softening during ripening. In addition, the early expression of Cel2 in 
      green fruit, well before significant softening begins, suggests that the product 
      of this gene may also be involved in processes other than fruit softening, e.g. 
      cell wall expansion.
FAU - Llop-Tous, I
AU  - Llop-Tous I
AD  - Departmento de Agrobiologia, Centro de Investigacion y Desarrollo, Consejo 
      Superior de Investigaciones Cientificas, Jordi Girona, 18-26, 08034 Barcelona, 
      Spain.
FAU - Dominguez-Puigjaner, E
AU  - Dominguez-Puigjaner E
FAU - Palomer, X
AU  - Palomer X
FAU - Vendrell, M
AU  - Vendrell M
LA  - eng
SI  - GENBANK/AF051346
SI  - GENBANK/AF054615
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA, Plant)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Plant)
RN  - EC 3.2.1.4 (Cellulase)
RN  - EC 3.2.1.91 (Cellulose 1,4-beta-Cellobiosidase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Cellulase/*genetics
MH  - Cellulose 1,4-beta-Cellobiosidase
MH  - Cloning, Molecular
MH  - DNA Primers/genetics
MH  - DNA, Complementary/*genetics
MH  - DNA, Plant/*genetics
MH  - Fruit/*enzymology/*genetics/growth & development
MH  - Genes, Plant
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA, Messenger/genetics/metabolism
MH  - RNA, Plant/genetics/metabolism
MH  - Sequence Homology, Amino Acid
PMC - PMC32027
EDAT- 1999/04/10 00:00
MHDA- 1999/04/10 00:01
PMCR- 2000/04/01
CRDT- 1999/04/10 00:00
PHST- 1999/04/10 00:00 [pubmed]
PHST- 1999/04/10 00:01 [medline]
PHST- 1999/04/10 00:00 [entrez]
PHST- 2000/04/01 00:00 [pmc-release]
AID - 10.1104/pp.119.4.1415 [doi]
PST - ppublish
SO  - Plant Physiol. 1999 Apr;119(4):1415-22. doi: 10.1104/pp.119.4.1415.