PMID- 10200369
OWN - NLM
STAT- Publisher
LR  - 20191120
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 16
IP  - 2
DP  - 1999 Apr 10
TI  - [Using the method of two-color fluorescence in situ hybridization to detect 
      aneuploidy of human sperms].
PG  - 116-118
AB  - OBJECTIVE: To develope the method of two-color fluorescence in situ hybridization 
      (FISH) assay and use it for detecting the aneuploidy frequency of human sperm 
      chromosome. METHODS: Sperm sample was washed three times and the slides were 
      prepared. The sperm heads were decondensed with dithiothreitol (DTT) and lithium 
      diiodosalicylate (LIS). Then, the sperm nuclei were hybridized with biotin 
      labeled alpha satellite X chromosome DNA probe (DXZ1) and digoxigenin labeled 
      alpha satellite Y chromosome DNA probe (DYZ3). The hybridization signals were 
      detected with CY3-Streptavidin, goat antistrepavidin for biotin labeled probe and 
      with mouse antidigoxigenin, rabbit antimouse-FITC for digoxigenin labeled probe. 
      RESULTS: Under the Nikon fluorescence microscope, the hybridization signals in 
      the sperm heads were clearly visible. The sperm with one red hybridization signal 
      was X chromosome sperm (X sperm), and the sperm with one green hybridization 
      signal in the sperm head was Y chromosome sperm (Y sperm). In the case of two 
      hybridization signals in one sperm head, the sperm should be a numerical abnormal 
      one. By using two color FISH with one euchromosome probe and one sex chromosome 
      probe, the sperm with same color of two hybridization signals in one sperm head 
      could be identified as aneuploidy sperm or diploid sperm. CONCLUSION: The two 
      color FISH assay may be used to detect the aneuploidy frequency of human sperms 
      that were exposed to mutagents and environmental potential aneuoploidogenic 
      agents.
FAU - Zheng, L
AU  - Zheng L
AD  - Laboratory of Genetic Toxicology, Sun Yat-Sen University of Medical Sciences, 
      Guangzhou, 510089, P. R. China. zhenglk@gzsums.edu.cn
FAU - Liu, S
AU  - Liu S
FAU - Deng, L
AU  - Deng L
FAU - Zhang, Q
AU  - Zhang Q
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
EDAT- 1999/04/14 00:00
MHDA- 1999/04/14 00:00
CRDT- 1999/04/14 00:00
PHST- 1999/04/14 00:00 [pubmed]
PHST- 1999/04/14 00:00 [medline]
PHST- 1999/04/14 00:00 [entrez]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 1999 Apr 10;16(2):116-118.