PMID- 10202982 OWN - NLM STAT- MEDLINE DCOM- 19990622 LR - 20190724 IS - 0022-510X (Print) IS - 0022-510X (Linking) VI - 162 IP - 2 DP - 1999 Jan 15 TI - Increased expression of glial cell line-derived neurotrophic factor mRNA in muscle biopsies from patients with amyotrophic lateral sclerosis. PG - 169-73 AB - The expression of glial cell line-derived neurotrophic factor (GDNF) mRNA and brain-derived neurotrophic factor (BDNF) mRNA were studied in muscle biopsies from five patients with amyotrophic lateral sclerosis (ALS), six patients with other neuromuscular diseases and eight healthy control persons. All five patients with ALS had higher GDNF mRNA expressions in their biopsies than the healthy control group (almost a three fold increase). Among the other patients only one, who had a rapidly progressing toxic polyneuropathy, showed a GDNF mRNA expression above those of the controls. The BDNF mRNA expressions in the biopsies from the ALS patients were in the same range as those from the healthy controls, although the mean value of the ALS patients was higher. The only biopsy that showed a markedly higher BDNF mRNA expression was taken from one patient with progressive muscular atrophy. These results suggest that increased GDNF mRNA expression in muscle is an unspecific response to ongoing denervation and that this response is maintained in ALS, at least temporarily. If increased GDNF mRNA in muscle proves to be a constant finding in ALS the rationale for the use of GDNF as a therapeutic agent in ALS must be questioned. FAU - Grundstrom, E AU - Grundstrom E AD - Department of Neuroscience, Neurology, University Hospital, Uppsala, Sweden. eva.grundstrom@neurologi.uu.se FAU - Askmark, H AU - Askmark H FAU - Lindeberg, J AU - Lindeberg J FAU - Nygren, I AU - Nygren I FAU - Ebendal, T AU - Ebendal T FAU - Aquilonius, S M AU - Aquilonius SM LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Netherlands TA - J Neurol Sci JT - Journal of the neurological sciences JID - 0375403 RN - 0 (GDNF protein, human) RN - 0 (Glial Cell Line-Derived Neurotrophic Factor) RN - 0 (Nerve Growth Factors) RN - 0 (Nerve Tissue Proteins) RN - 0 (RNA, Messenger) SB - IM MH - Adult MH - Aged MH - Amyotrophic Lateral Sclerosis/*metabolism/pathology MH - Biopsy MH - Case-Control Studies MH - Female MH - Glial Cell Line-Derived Neurotrophic Factor MH - Humans MH - Male MH - Middle Aged MH - Muscle, Skeletal/*metabolism/pathology MH - Nerve Growth Factors/*genetics MH - Nerve Tissue Proteins/*genetics MH - RNA, Messenger/*biosynthesis EDAT- 1999/04/15 00:00 MHDA- 1999/04/15 00:01 CRDT- 1999/04/15 00:00 PHST- 1999/04/15 00:00 [pubmed] PHST- 1999/04/15 00:01 [medline] PHST- 1999/04/15 00:00 [entrez] AID - S0022510X98003335 [pii] AID - 10.1016/s0022-510x(98)00333-5 [doi] PST - ppublish SO - J Neurol Sci. 1999 Jan 15;162(2):169-73. doi: 10.1016/s0022-510x(98)00333-5.