PMID- 10203333
OWN - NLM
STAT- MEDLINE
DCOM- 19990603
LR  - 20190718
IS  - 0959-4965 (Print)
IS  - 0959-4965 (Linking)
VI  - 10
IP  - 2
DP  - 1999 Feb 5
TI  - Expression and modulation of matrix metalloproteinase-2 and tissue inhibitors of 
      metalloproteinases in human embryonic CNS stem cells.
PG  - 345-51
AB  - The expression and regulation of matrix metalloproteinases (MMPs) and tissue 
      inhibitors of MMPs (TIMPs) in neuroectodermal precursor cells is undocumented. We 
      report the presence of MMP-2, but no MMP-9, and of all the four known TIMPs in 
      neuroepithelial stem cells isolated from the human CNS. The expression of TIMP-1, 
      TIMP-2 and TIMP-3 was unchanged following stem cells differentiation into neurons 
      and glia. In contrast, while MMP-2 and TIMP-4 were localized to both stem and 
      mature CNS cells, their levels of expression were substantially reduced in the 
      latter. TIMP-4 showed a 23-fold reduction in media conditioned by differentiated 
      cells compared with stem cell-conditioned media, reflecting a 6-fold decrease in 
      mRNA expression. Interestingly, TIMP-4 also differed from the other TIMPs in that 
      it was cell-associated in the stem cells, where this fraction remained unchanged 
      upon differentiation. Hence, regulation of selective MMPs and TIMPs occurs during 
      differentiation of human neural precursors suggesting that MMP-2 and TIMP-4 in 
      particular may perform regulatory roles in the developing CNS.
FAU - Frolichsthal-Schoeller, P
AU  - Frolichsthal-Schoeller P
AD  - Department of Medical Biochemistry, University of Calgary, Canada.
FAU - Vescovi, A L
AU  - Vescovi AL
FAU - Krekoski, C A
AU  - Krekoski CA
FAU - Murphy, G
AU  - Murphy G
FAU - Edwards, D R
AU  - Edwards DR
FAU - Forsyth, P
AU  - Forsyth P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neuroreport
JT  - Neuroreport
JID - 9100935
RN  - 0 (RNA, Messenger)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - EC 3.4.24.- (Collagenases)
RN  - EC 3.4.24.- (Gelatinases)
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Cells, Cultured
MH  - Collagenases/genetics/metabolism
MH  - Diencephalon/*embryology
MH  - Embryo, Mammalian/cytology/metabolism
MH  - Gelatinases/genetics/*metabolism
MH  - Humans
MH  - Matrix Metalloproteinase 2
MH  - Matrix Metalloproteinase 9
MH  - Metalloendopeptidases/genetics/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Stem Cells/*metabolism
MH  - Tissue Inhibitor of Metalloproteinases/genetics/*metabolism
EDAT- 1999/04/15 00:00
MHDA- 1999/04/15 00:01
CRDT- 1999/04/15 00:00
PHST- 1999/04/15 00:00 [pubmed]
PHST- 1999/04/15 00:01 [medline]
PHST- 1999/04/15 00:00 [entrez]
AID - 10.1097/00001756-199902050-00025 [doi]
PST - ppublish
SO  - Neuroreport. 1999 Feb 5;10(2):345-51. doi: 10.1097/00001756-199902050-00025.