PMID- 10206332
OWN - NLM
STAT- MEDLINE
DCOM- 19990610
LR  - 20190717
IS  - 0003-9969 (Print)
IS  - 0003-9969 (Linking)
VI  - 44
IP  - 2
DP  - 1999 Feb
TI  - Expression of hepatocyte growth factor/scatter factor and c-Met in human dental 
      papilla and fibroblasts from dental papilla.
PG  - 135-47
AB  - Hepatocyte growth factor/scatter factor (HGF/SF), a broad-spectrum and 
      multifunctional cytokine, is essential for the development of tissues including 
      tooth. Here it was found that the HGF/SF content of human dental papillae 
      obtained from 8 to 16-year-old individuals decreased significantly with age. 
      Cultured fibroblasts prepared from the dental papillae of individuals of 
      different ages produced HGF/SF at almost the same rate, but the sensitivities of 
      the cells to interleukin-1alpha and tumour necrosis factor-alpha for the 
      production of HGF/SF increased with age. Generally, mesenchymal cells such as 
      fibroblasts produce HGF/SF but do not express c-Met, a receptor for HGF/SF, yet 
      fibroblasts in dental papilla and cultured fibroblasts prepared from dental 
      papilla did express c-Met, as determined by immunohistochemistry, in situ 
      hybridization and reverse transcription-polymerase chain reaction. Recombinant 
      human [125I]iodo-HGF/SF specifically bound to cell-surface macromolecules with a 
      mol. wt of 146,000, which is the same as that of the beta-subunit of c-Met. The 
      physiological role of c-Met on fibroblasts in dental papilla is unknown, but the 
      addition of 2 ng of HGF/SF per ml to the culture medium significantly stimulated 
      DNA synthesis in the cells, as determined by pulse labelling with [3H]thymidine. 
      Exogenous HGF/SF also stimulated secretion by the cells of vascular endothelial 
      growth factor, a cytokine that induces blood vessel-formation. These results 
      suggest that HGF/SF may be involved in tooth development via autocrine 
      mechanisms.
FAU - Kajihara, T
AU  - Kajihara T
AD  - Department of Biochemistry, Kagoshima University Dental School, Japan.
FAU - Ohnishi, T
AU  - Ohnishi T
FAU - Arakaki, N
AU  - Arakaki N
FAU - Semba, I
AU  - Semba I
FAU - Daikuhara, Y
AU  - Daikuhara Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (Endothelial Growth Factors)
RN  - 0 (Interleukin-1)
RN  - 0 (Iodine Radioisotopes)
RN  - 0 (Lymphokines)
RN  - 0 (Radiopharmaceuticals)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (Vascular Endothelial Growth Factors)
RN  - 10028-17-8 (Tritium)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
RN  - VC2W18DGKR (Thymidine)
SB  - IM
MH  - Adolescent
MH  - Aging/genetics/metabolism
MH  - Autocrine Communication/physiology
MH  - Cells, Cultured
MH  - Child
MH  - DNA/biosynthesis
MH  - Dental Papilla/cytology/*metabolism
MH  - Endothelial Growth Factors/biosynthesis
MH  - Fibroblasts/cytology/*metabolism
MH  - Gene Expression Regulation
MH  - Hepatocyte Growth Factor/*analysis/genetics
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Interleukin-1/pharmacology
MH  - Iodine Radioisotopes
MH  - Lymphokines/biosynthesis
MH  - Mesoderm/cytology/metabolism
MH  - Molecular Weight
MH  - Neovascularization, Physiologic
MH  - Odontogenesis/physiology
MH  - Polymerase Chain Reaction
MH  - Proto-Oncogene Proteins c-met/*analysis/genetics
MH  - Radiopharmaceuticals
MH  - Thymidine/metabolism
MH  - Tritium
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - Vascular Endothelial Growth Factor A
MH  - Vascular Endothelial Growth Factors
EDAT- 1999/04/17 00:00
MHDA- 1999/04/17 00:01
CRDT- 1999/04/17 00:00
PHST- 1999/04/17 00:00 [pubmed]
PHST- 1999/04/17 00:01 [medline]
PHST- 1999/04/17 00:00 [entrez]
AID - S0003996998001010 [pii]
AID - 10.1016/s0003-9969(98)00101-0 [doi]
PST - ppublish
SO  - Arch Oral Biol. 1999 Feb;44(2):135-47. doi: 10.1016/s0003-9969(98)00101-0.