PMID- 10206798
OWN - NLM
STAT- MEDLINE
DCOM- 19990413
LR  - 20190513
IS  - 0022-2585 (Print)
IS  - 0022-2585 (Linking)
VI  - 36
IP  - 2
DP  - 1999 Mar
TI  - Fluorescence in situ hybridization landmarks for chromosomes of culicoides 
      variipennis (Diptera: Ceratopogonidae).
PG  - 171-5
AB  - Because the three chromosomes of Culicoides variipennis (Coquillett) are 
      morphologically indistinguishable, physical landmarks were needed so that the 
      chromosomes can be identified uniquely and oriented unambiguously before 
      initiating the construction of a physical map based on FISH (fluorescence in situ 
      hybridization). When repetitive sequence clones p1887 and K8.1a8 were labeled 
      with digoxigenin and probe K8a.2G2 was labeled with biotin-11-dUTP and 
      digoxigenin, the 3 chromosomes could be differentiated unambiguously when 
      visualized with specific band-pass filters. This provided the basis for C. 
      variipennis FISH landmark probes that enabled the identification and orientation 
      of all 3 pairs of chromosomes. Using this multicolor FISH labeling strategy, 
      probes that provide unique landmarks for the C. variipennis FISH physical map 
      have been found and may be used in all FISH reactions where an unknown probe is 
      placed to metaphase chromosomes of C. variipennis. A physical map of the C. 
      variipennis genome will provide the foundation for map-based positional cloning 
      of the gene(s) that control vector competence for the bluetongue viruses.
FAU - Nunamaker, R A
AU  - Nunamaker RA
AD  - Arthropod-Borne Animal Diseases Research Laboratory, USDA-ARS, Laramie, WY 82071, 
      USA.
FAU - Brown, S E
AU  - Brown SE
FAU - Knudson, D L
AU  - Knudson DL
LA  - eng
GR  - AI 34337/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Med Entomol
JT  - Journal of medical entomology
JID - 0375400
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Ceratopogonidae/*genetics
MH  - *Genes, Insect
MH  - In Situ Hybridization, Fluorescence/*methods
EDAT- 1999/04/17 00:00
MHDA- 1999/04/17 00:01
CRDT- 1999/04/17 00:00
PHST- 1999/04/17 00:00 [pubmed]
PHST- 1999/04/17 00:01 [medline]
PHST- 1999/04/17 00:00 [entrez]
AID - 10.1093/jmedent/36.2.171 [doi]
PST - ppublish
SO  - J Med Entomol. 1999 Mar;36(2):171-5. doi: 10.1093/jmedent/36.2.171.