PMID- 10209239
OWN - NLM
STAT- MEDLINE
DCOM- 19990526
LR  - 20190831
IS  - 0165-3806 (Print)
IS  - 0165-3806 (Linking)
VI  - 114
IP  - 1
DP  - 1999 Apr 12
TI  - Growth factors effects on the expression of morphological and biochemical 
      properties of avian embryonic sympathetic cells. Emphasis on NGF.
PG  - 27-36
AB  - Growth factors are known to be important agents in the differentiation and 
      modulation of neuronal phenotypes. We have analyzed the effect of several growth 
      factors on the modulation of morphological and biochemical properties of avian 
      embryonic sympathetic neurons. The growth factors studied include: nerve growth 
      factor (NGF), neurotrophin-3 (NT-3), brain derived neurotrophic factor (BDNF), 
      ciliary neurotrophic factor (CNTF), basic fibroblast growth factor (bFGF) and 
      transforming growth factor beta-1 (TGF-beta1). Morphological properties were 
      analyzed by immunocytochemistry to neurofilament proteins and visualization of 
      fibers after glyoxylic acid-induced fluorescence. Biochemical modulation was 
      determined by radioimmunoanalysis for the peptides enkephalin (ENK), somatostatin 
      (SS) and neuropeptide Y (NPY) and by HPLC-electrochemistry quantification of 
      catecholamines. Similar to previous results using chromaffin cell cultures [R. 
      Ramirez-Ordonez, J.E. Garcia-Arraras, Peptidergic, catecholaminergic and 
      morphological properties of avian chromaffin cells are modulated distinctively by 
      growth factors, Dev. Brain Res., 87 (1995) 160-171], we found a dissociation in 
      the modulation of biochemical and morphological properties, however, the effect 
      of specific factors differed between the chromaffin and sympathetic cultures. We 
      have focused on NGF to analyze its effect on the sympathetic peptide phenotypes 
      and its lack of an effect on the chromaffin cell peptide phenotypes. The results 
      presented here, establish interesting differences between chromaffin cells and 
      sympathetic neurons that are of importance to studies of cell lineage and 
      differentiation.
CI  - Copyright 1999 Elsevier Science B.V.
FAU - Ramirez-Ordonez, R
AU  - Ramirez-Ordonez R
AD  - Department of Biology, University of Puerto Rico, Box 23360, Rio Piedras Campus, 
      Rio Piedras, Puerto Rico 00931-3360, USA.
FAU - Barreto-Estrada, J L
AU  - Barreto-Estrada JL
FAU - Garcia-Arraras, J E
AU  - Garcia-Arraras JE
LA  - eng
GR  - RR-8102-18/RR/NCRR NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res Dev Brain Res
JT  - Brain research. Developmental brain research
JID - 8908639
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Catecholamines)
RN  - 0 (Ciliary Neurotrophic Factor)
RN  - 0 (Enkephalins)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neuropeptide Y)
RN  - 0 (Neurotrophin 3)
RN  - 0 (Transforming Growth Factor beta)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 51110-01-1 (Somatostatin)
SB  - IM
MH  - Adrenal Glands/cytology
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Catecholamines/physiology
MH  - Cell Differentiation/drug effects
MH  - Cell Division/drug effects
MH  - Chick Embryo
MH  - Chromaffin Cells/chemistry/drug effects/physiology
MH  - Ciliary Neurotrophic Factor
MH  - Dose-Response Relationship, Drug
MH  - Enkephalins/analysis
MH  - Fibroblast Growth Factor 2/pharmacology
MH  - Nerve Growth Factors/*pharmacology
MH  - Nerve Tissue Proteins/pharmacology
MH  - Neurons/chemistry/*cytology/*drug effects
MH  - Neuropeptide Y/analysis
MH  - Neurotrophin 3
MH  - Somatostatin/analysis
MH  - Sympathetic Nervous System/chemistry/*cytology/*drug effects
MH  - Transforming Growth Factor beta/pharmacology
EDAT- 1999/04/21 00:00
MHDA- 1999/04/21 00:01
CRDT- 1999/04/21 00:00
PHST- 1999/04/21 00:00 [pubmed]
PHST- 1999/04/21 00:01 [medline]
PHST- 1999/04/21 00:00 [entrez]
AID - S0165380699000127 [pii]
AID - 10.1016/s0165-3806(99)00012-7 [doi]
PST - ppublish
SO  - Brain Res Dev Brain Res. 1999 Apr 12;114(1):27-36. doi: 
      10.1016/s0165-3806(99)00012-7.